Advertisement

Actin pp 3-12 | Cite as

Vibrational Modes of G-Actin

  • Monique M. Tirion
  • Daniel ben-Avraham
  • Kenneth C. Holmes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 358)

Abstract

The determination of the atomic structure of g-actin (Kabsch et al., 1990, see Fig. 1) allowed the development of an atomic model for f-actin (Holmes et al., 1990). The structure of f-actin was deduced from x-ray diffraction patterns from bundles of aligned actin filaments, using the known helical symmetry of the filament and keeping the atomic structure of the monomer fixed. The model of f-actin was obtained, therefore, using only four structural parameters: three rotational and one radial degree of freedom. The solution thus obtained is unique, and achieved an R-factor of.22; an extremely good fit with such few parameters.

Keywords

Normal Mode Slow Mode Normal Mode Analysis Binding Loop Hinge Point 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. ben-Avraham, D., 1993, Vibrational normal-mode spectrum of globular proteins, Phys. Rev. B 47: 14559.CrossRefGoogle Scholar
  2. Brooks, B & Karplus, B., 1983, Harmonic dynamics of proteins: Normal modes and fluctuations in bovine pancreatic trypsin inhibitor, Biophysics 80: 6571.Google Scholar
  3. Diamond, R., 1990, On the use of normal modes in thermal parameter refinement: theory and application to bovine pancreatic trypsin inhibitor, Acta Cryst. A46: 425.Google Scholar
  4. Egelman E. H., Francis, N. & DeRosier, D. J., 1982, F-actin is a helix with a random variable twist, Nature 298: 131.PubMedCrossRefGoogle Scholar
  5. Flaherty K. M., McKay, D., Kabsch, W., & Holmes, K. C., 1991, Three-dimensional structure of the ATPase fragment of a 70kD heat-shock cognate protein, Proc. Natl. Acad. Sci. USA 88: 5041.PubMedCrossRefGoogle Scholar
  6. Goldstein, H., 1950, “Classical Mechanics”, Addison-Wesley, Reading, Massachusetts.Google Scholar
  7. Hegyi G., Michel H., Shabanowitz J., Hunt D. F., Chatterjee N., Healy-Louie, G. and Elzinga, M., 1992, Gln-41 is intermolecularly cross-linked to lys-113 in f-actin by N-(4-azidobenzoyl)-putresine. Protein Science, 1: 132.PubMedCrossRefGoogle Scholar
  8. Holmes, K. C., Popp, D., Gebhard, W., Kabsch, W., 1990, Atomic model of the actin filament, Nature 347: 44.PubMedCrossRefGoogle Scholar
  9. Kabsch, W., Mannherz H. G., Suck, D., Pai, E., Holmes, K. C., 1990, Atomic structure of the actin: DNase I complex, Nature 347: 37.PubMedCrossRefGoogle Scholar
  10. Levitt, M., 1983, Molecular dynamics of native protein. I. Computer simulation of trajectories, J. Mol. Biol. 168: 595.PubMedCrossRefGoogle Scholar
  11. Levitt M., Sander, C. and Stern, P. S., 1985, Protein normal-mode dynamics: trypsin inhibitor, crambin, ribonuclease and lysozyme, J. Mol. Biol. 181: 423.PubMedCrossRefGoogle Scholar
  12. Millonig, R., Siitterlin, R., Engel, A., Pollard, T. D., Aebi, U., 1989, The ‘lateral slipping’ model of F-actin filaments, in: “Springer Series In Biophysics Vol. 3, Cytoskeletal and Extracellular Proteins” U. Aebi and A. Engel, eds., Springer-Verlag, Heidelberg Google Scholar
  13. Orlova, A. and Egelman, E. H., 1993, A conformational change in the actin subunit can change the flexibility of the actin filament. J. Mol. Biol. In press.Google Scholar
  14. Steitz T. A., Anderson, W. F., Fletterick, R. J. & Anderson, C. M., 1977, High resolution crystal structures of yeasts hexokinase complexes with substrates, activators, and inhibitors. Evidence of an allosteric control site, J. Biol.Chem. 252: 4494.PubMedGoogle Scholar
  15. Tirion M. M., & ben-Avraham, D., 1993, Normal mode analysis of g-actin, J. Mol. Biol. 230: 186.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Monique M. Tirion
    • 1
  • Daniel ben-Avraham
    • 1
  • Kenneth C. Holmes
    • 2
  1. 1.Physics DepartmentClarkson UniversityPotsdamUSA
  2. 2.Max-Planck-Institute for Medical ResearchHeidelbergGermany

Personalised recommendations