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Culturing Carotid Body Cells of Adult Cats

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 360))

Abstract

Recently patch clamp techniques or optical fluorometric techniques have been applied to freshly dissociated or cultured carotid body glomus cells to understand chemosensory mechanisms of the carotid body. Results were not consistent. The inconsistency of the available data may result from various reasons. Firstly, the studied cells might have been heterogeneous due to the difficulty of differentiating between dissociated glomus cells and sheath cells. Secondly, culture conditions may alter characteristics of the glomus cells. Thirdly, differences in the age and species used to generate the available data make the interpretation more difficult. The purpose of this study is to standardise our culture techniques to overcome these difficulties.

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References

  • Banker, G. &Goslin, K. (1991). Culturing nerve cells. Cambridge, MA: The MIT press

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© 1994 Springer Science+Business Media New York

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Shirahata, M., Schofield, B., Chin, B.Y., Guilarte, T.R. (1994). Culturing Carotid Body Cells of Adult Cats. In: O’Regan, R.G., Nolan, P., McQueen, D.S., Paterson, D.J. (eds) Arterial Chemoreceptors. Advances in Experimental Medicine and Biology, vol 360. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2572-1_19

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  • DOI: https://doi.org/10.1007/978-1-4615-2572-1_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6099-5

  • Online ISBN: 978-1-4615-2572-1

  • eBook Packages: Springer Book Archive

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