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Direct cDNA Selection Using Human and Mouse cDNAs: Application to Xq13.3 Chromosomal Region

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Identification of Transcribed Sequences

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Abstract

The capability of the direct cDNA selection technique to work in heterologous conditions is described. A human YAC (4551) positive for DXS56 from the Xq13.3 DXS56-PGK1 region was successfully scanned for the presence of conserved expressed sequences exploiting a mouse brain cDNA library. Clones from a known, conserved gene xnp were identified. In addition, one new gene was isolated. The sequence comparison between human and mouse XNP/xnp selected clones revealed at least 75% homology on the nucleotide level. Although the efficiency is limited by the level of sequence homology, the direct cDNA selection is applicable to heterologous conditions.

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Author information

Authors and Affiliations

  1. Institute of Molecular Physiology and Genetics, Department of Genetics, Slovak Academy of Sciences, Vlarska 5, 83334, Bratislava, Slovakia

    J. Gecz

  2. INSERM U242, 27 Boulevard J. Moulin, 13385, Marseille, Cedex 5, France

    L. Villard, A. M. Lossi & M. Fontes

Authors
  1. J. Gecz
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  2. L. Villard
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  3. A. M. Lossi
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  4. M. Fontes
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Editor information

Editors and Affiliations

  1. National Institute of Mental Health, Bethesda, Maryland, USA

    Ute Hochgeschwender

  2. Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado, USA

    Katheleen Gardiner

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© 1994 Springer Science+Business Media New York

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Gecz, J., Villard, L., Lossi, A.M., Fontes, M. (1994). Direct cDNA Selection Using Human and Mouse cDNAs: Application to Xq13.3 Chromosomal Region. In: Hochgeschwender, U., Gardiner, K. (eds) Identification of Transcribed Sequences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2562-2_9

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  • DOI: https://doi.org/10.1007/978-1-4615-2562-2_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6094-0

  • Online ISBN: 978-1-4615-2562-2

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