Abstract
The number of DNA clones to be manipulated and analysed in a variety of projects dealing with the analysis of complex genomes exceeds the potential of current methodology by orders of magnitude. By focussing first on the transcribed parts of the genome, i.e., by using cDNA or exon-trap libraries, it is possible to reduce the volume of the task considerably, presumably without sacrificing too much information. We present here an integrated series of mostly automated processes which together allow the isolation, amplification, arrayed spotting and analysis by oligonucleotide fingerprinting of > 100,000 cDNA clones in a few months with little operator involvement. The sequence information thus derived will be used to search databases for related sequences and to establish catalogues of expressed sequences. The technique is currently being applied to the analysis of cDNA derived from various human and mouse tissues and developmental stages. An expanded version of the process will allow us to analyse cDNA libraries from a range of representative human tissues, thereby giving us access to a significant fraction of the human genome.
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Meier-Ewert, S., Rothe, J., Mott, R., Lehrach, H. (1994). Establishing Catalogues of Expressed Sequences by Oligonucleotide Fingerprinting of cDNA Libraries. In: Hochgeschwender, U., Gardiner, K. (eds) Identification of Transcribed Sequences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2562-2_22
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DOI: https://doi.org/10.1007/978-1-4615-2562-2_22
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