Abstract
A novel strategy for rapid identification of unknown coding sequences from large genomic regions has been developed. It is based on selective in vitro recombination of genomic DNA and cDNA followed by Polymerase Chain Reaction. The technique involves generation of cDNA primers, by restriction digestion of cDNA libraries, that hybridize to their cognate genomic DNA sequences. These hybrids are chain elongated using the free 3’ end of the cDNA fragment as a primer, then PCR amplified using primers previously attached to genomic DNA and cDNA. Unknown coding sequences are clearly discernable as genomic cDNA chimeras.
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Jagadeeswaran, P., Odom, M.W., Boland, E.J. (1994). Novel Strategy for Isolating Unknown Coding Sequences from Genomic DNA by Generating Genomic-cDNA Chimeras. In: Hochgeschwender, U., Gardiner, K. (eds) Identification of Transcribed Sequences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2562-2_11
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DOI: https://doi.org/10.1007/978-1-4615-2562-2_11
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