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Urolithiasis 2 pp 101-103 | Cite as

Isolation and Characterization of Oxalate Decarboxylase From Human Intestine Bacteria

  • H. Ito
  • H. Hayashi
  • T. Kotake
  • Y. Yokoo
  • K. Yamamoto
  • T. Hara
  • T. Furukawa
  • Y. Nakagawa

Abstract

Allison et al reported the presence of an oxalate degrading microorganism, Oxalobacter formigenes, in the human colon1. They suggested that this microorganism decomposes oxalate in intestine and consequently decreases the amount of oxalate available for absorption. In order to study the degradation and absorption of oxalate in the intestine of human subjects, we isolated the oxalate decarboxylase from anaerobic bacteria obtained from human feces, and examined the biochemical properties of this enzyme.

Keywords

Human Feces Sodium Oxalate Human Intestine Bacterium Oxalate Decarboxylase Couple Enzyme System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    MJ Allison, HM Cook, DB Milne, S Gallagher and RV dayman, Oxalate degradation by gastrointestinal bacteria from humans, J Nutr 116: 455 (1986).PubMedGoogle Scholar
  2. 2.
    OH Lowry, NJ Rosebrough, AL Fan and RJ Randall, Protein measurement with the Folin phenol reagent, J Biol Chem 193: 265 (1951).PubMedGoogle Scholar
  3. 3.
    AT Andrews, in: “Electrophoresis”, 2nd Ed. Chapter 5 Clarendon Press, Oxford (1986).Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • H. Ito
    • 1
  • H. Hayashi
    • 1
  • T. Kotake
    • 1
  • Y. Yokoo
    • 2
  • K. Yamamoto
    • 2
  • T. Hara
    • 2
  • T. Furukawa
    • 3
  • Y. Nakagawa
    • 4
  1. 1.Department of UrologyTeikyo University School of Medicine Ichihara HospitalIchiharaJapan
  2. 2.Tokyo Research LaboratoriesKyowa Hakko Kogyo Co LtdMachidaJapan
  3. 3.Nutri-Quest, IncChesterfieldUK
  4. 4.Nephrology ProgramUniversity of ChicagoChicagoUSA

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