Urolithiasis 2 pp 351-353 | Cite as

Calcium Oxalate Crystallizing Properties of Macromolecules Released by Renal Tubular Cells in Vitro

  • D. K. Y. Shum
  • E. Liong


Urinary proteins and glycosaminoglycans (GAGs) have been shown to act diversely on the crystallization of calcium oxalate. In media where these macromolecules coexist as solubilized and immobilized forms, they are dually effective as promoters of nucleation and inhibitors of growth in the process of crystallization1, 2. Structural modifications of urinary proteins or structural differences of urinary GAGs can also influence crystallization3, 4. Micropuncture and renal clearance studies have shown that only a small proportion of plasma proteins and GAGs normally enter the tubular fluid via glomerular ultrafiltration, and escape tubular reabsorption to be excreted in the urine5. It follows that the majority of urinary GAGs, like the urinary proteins, are of renal origin. It is of interest therefore to develop an in vitro system to study renal tubular cell activities that release macromolecules that may influence crystallization of urinary calcium oxalate.


Calcium Oxalate Calcium Oxalate Monohydrate Basal Compartment Urinary GAGs Aminohexanoic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    AA Campbell, A Ebrahimpour, L Perez, SA Smesko and GH Nancollas, The dual role of polyelectrolytes and proteins as mineralization promoters and inhibitors of calcium oxalate monohydrate. Calcif Tissue Int 45: 122 (1989).PubMedCrossRefGoogle Scholar
  2. 2.
    MD Gohel, DKY Shum and MK Li, The dual effect of urinary macromolecules on the crystallization of calcium oxalate endogenous in urine, Urol Res 20: 13 (1992).PubMedCrossRefGoogle Scholar
  3. 3.
    B Hess, Y Nakagawa, J Parks and F Coe, Molecular abnormality of Tamm-Horsfall glycoprotein in calcium oxalate nephrolithiasis, Am J Physiol 260: F569 (1991).PubMedGoogle Scholar
  4. 4.
    MD Gohel and DKY Shum, Urinary glycosaminoglycans differ in their calcium oxalate crystallization activities, in: this volume.Google Scholar
  5. 5.
    DKY Shum, C Baylis and JE Scott, A micropuncture and renal clearance study in the rat of the urinary excretion of heparin, chondroitin sulphate and metabolic breakdown products of connective tissue proteoglycans, Clin Sci 67: 205 (1984).PubMedGoogle Scholar
  6. 6.
    PYD Wong, Mechanism of adrenergic stimulation of anion secretion in cultured rat epididymal epithelium, Am J Physiol 254: F121 (1988).PubMedGoogle Scholar
  7. 7.
    M Taub and G Sato, Growth of functional primary cultures of kidney epithelial cells in defined medium, J Cell Physiol 105: 369 (1980).PubMedCrossRefGoogle Scholar
  8. 8.
    T Bitter and H Muir, A modified uronic acid carbazole reaction, Anal Biochem 4: 330 (1962)PubMedCrossRefGoogle Scholar
  9. 9.
    DJ Carey and MS Todd, A cytoskeleton-associated basement membrane heparan sulphate proteoglycan in Schwann cells, J Biol Chem 261: 7518 (1986).PubMedGoogle Scholar
  10. 10.
    H Saito, T Yamagata and S Suzuki, Enzymatic methods for the determination of small quantities of isomeric chondroitin sulphates, J Biol Chem 243: 1536 (1968).PubMedGoogle Scholar
  11. 11.
    KA Edyvane, RJ Ryall, RD Mazzachi and VR Marshall, The effect of serum on the crystallization of calcium oxalate in whole human urine: inhibition disguised as apparent promotion, Urol Res 15: 87 (1987).PubMedCrossRefGoogle Scholar
  12. 12.
    JE Shively and HE Conrad, Formation of anhydrosugars in the chemical depolymerization of heparin, Biochem 15: 3932 (1976).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • D. K. Y. Shum
    • 1
  • E. Liong
    • 1
  1. 1.Department of Biochemistry, Faculty of MedicineThe University of Hong KongHong Kong

Personalised recommendations