Urolithiasis 2 pp 351-353 | Cite as

Calcium Oxalate Crystallizing Properties of Macromolecules Released by Renal Tubular Cells in Vitro

  • D. K. Y. Shum
  • E. Liong

Abstract

Urinary proteins and glycosaminoglycans (GAGs) have been shown to act diversely on the crystallization of calcium oxalate. In media where these macromolecules coexist as solubilized and immobilized forms, they are dually effective as promoters of nucleation and inhibitors of growth in the process of crystallization1, 2. Structural modifications of urinary proteins or structural differences of urinary GAGs can also influence crystallization3, 4. Micropuncture and renal clearance studies have shown that only a small proportion of plasma proteins and GAGs normally enter the tubular fluid via glomerular ultrafiltration, and escape tubular reabsorption to be excreted in the urine5. It follows that the majority of urinary GAGs, like the urinary proteins, are of renal origin. It is of interest therefore to develop an in vitro system to study renal tubular cell activities that release macromolecules that may influence crystallization of urinary calcium oxalate.

Keywords

Cellulose Crystallization Heparin Electrophoresis Barium 

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • D. K. Y. Shum
    • 1
  • E. Liong
    • 1
  1. 1.Department of Biochemistry, Faculty of MedicineThe University of Hong KongHong Kong

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