Increased Hepatic Oxalate Production in Rats Treated With Clofibrate

  • A. M. Rofe
  • R. A. J. Conyers
  • R. Bais


Peroxisomes are thought to play an important role in hepatic metabolism1, 2. In oxalate stone disease where urinary oxalate is derived mainly from endogenous metabolic processes3, peroxisomes are important in that they are a site of oxalate production4 with glycollate oxidase, amino acid oxidase, glyoxylate:alanine aminotransferases and catalase being present in these organelles2, 5–7. The hypolipidaemic drug, clofibrate, causes both peroxisomal proliferation and alterations in the activity of enzymes located in these organelles, particularly catalase1, 8, 9. Clofibrate therefore offers a means of better understanding the regulation of hepatic oxalate production. In this study we describe the effect of clofibrate administration on the changes in enzyme activities and the corresponding effects on oxalate production in hepatocytes isolated from clofibrate-treated rats.


Urinary Oxalate Glycollate Oxidase Hepatocyte Suspension Amino Acid Oxidase Hypolipidaemic Drug 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    PB Lazarow, Peroxisomes in: “The Liver: Biology and Pathology” 2nd Edit Eds IM Arias, WB Jakoby, H Popper, D Schachter, DA Shafritz, Raven Press Ltd NY. p241 (1988).Google Scholar
  2. 2.
    NE Tolbert, Metabolic pathways in peroxisomes and glyoxysomes, Ann Rev Biochem 50: 133 (1981).PubMedCrossRefGoogle Scholar
  3. 3.
    R Bais, AM Rofe and RAJ Conyers, The inhibition of metabolic oxalate production by sulfhydryl compounds, J Urol 145: 1302 (1991).PubMedGoogle Scholar
  4. 4.
    DA Gibbs, S Hauschildt and RWE Watts, Glyoxylate oxidation in rat liver and kidney, J Biochem 82: 221 (1977).PubMedGoogle Scholar
  5. 5.
    LL Liao, and KE Richardson, The inhibition of oxalate biosynthesis in isolated perfused rat liver by DL-phenyllactate and n-heptanoate, Arch Biochem Biophys 154: 68 (1973).PubMedCrossRefGoogle Scholar
  6. 6.
    E McGroarty, B Hsieh, DM. Wied, R Gee, and NE Tolbert, Alpha-hydroxy acid oxidation by peroxisomes, Arch Biochem Biophys 161: 194 (1974).CrossRefGoogle Scholar
  7. 7.
    A Hand, Ultrastructural localisation of L-hydroxy acid oxidase in rat liver peroxisomes, Histochem 41: 196 (1975).CrossRefGoogle Scholar
  8. 8.
    R Hess, W Staubli and W Riess, Nature of the hepatomegalic effect produced by ethyl-chlorophenoxyisobutyrate in the rat, Nature 208: 856 (1965).PubMedCrossRefGoogle Scholar
  9. 9.
    H Hayashi, T Suga and S Niinobe, Studies on peroxisomes, V Effect of ethyl-p-chlorophenoxyisobutyrate on the centrifugal behaviour of rat liver peroxisomes, J Biochem 77: 1199 (1975).PubMedGoogle Scholar
  10. 10.
    AM Rofe, AH Chalmers and JB Edwards, [14C] oxalate synthesis from [U14C] glyoxylate and [114C] glycollate in isolated rat hepatocytes, Biochem Med 16: 277 (1976).PubMedCrossRefGoogle Scholar
  11. 11.
    AM Rofe, HM James, R Bais, JB Edwards and RAJ Conyers, The production of [14C] oxalate during the metabolism of [14C] carbohydrates in isolated rat hepatocytes, Aust J Exp Biol Med Sci 58: 103 (1980).PubMedCrossRefGoogle Scholar
  12. 12.
    HU Bergmeyer, and E Bernt, in “Methods of Enzymatic Analysis”, HU Bergmeyer, ed, Acad Press NY. p 846 (1963).Google Scholar
  13. 13.
    F Leighton, B Poole, H Beaufay, P Baudhin, JW Cofey, S Fowler & C DeDuve, The large scale separation of peroxisomes, mitochondria and lysosomes from the liver of rats injected with triton WR-1339, J Cell Biol 37: 482 (1968).PubMedCrossRefGoogle Scholar
  14. 14.
    D Zakim, RS Paradini and RH Herman, Effect of clofibrate (ethylchlorophenoxyisobutyrate) feeding on glycolytic and lipogenic enzymes and hepatic glycogen synthesis in the rat, Biochem Pharmacol 19: 305 (1970).PubMedCrossRefGoogle Scholar
  15. 15.
    JV O’Fallon and RW Brosemer, Cellular localization of ketoglutarate: glyoxylate carboligase in rat tissues, Biochim Biophys Acta 499: 321 (1977).PubMedCrossRefGoogle Scholar
  16. 16.
    B Halliwell and VS Butt, Oxidative decarboxylation of glycollate and glyoxylate by leaf peroxisomes, Biochem J 138: 217 (1974).PubMedGoogle Scholar
  17. 17.
    A Boveris, N Oshino and B Chance, The cellular production of hydrogen peroxide, Biochem J 128: 617 (1972).PubMedGoogle Scholar
  18. 18.
    B Poole, Diffusion effects in the metabolism of hydrogen peroxide by rat liver peroxisomes, J Theor Biol 51: 149 (1975).PubMedCrossRefGoogle Scholar
  19. 19.
    R Bais, AM Rofe and RAJ Conyers, Investigations into the effect of glyoxylate decarboxylation and transamination on oxalate formation in the rat, Nephron 57: 460 (1991).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • A. M. Rofe
    • 1
  • R. A. J. Conyers
    • 2
  • R. Bais
    • 1
  1. 1.Division of Clinical ChemistryInstitute of Medical & Veterinary ScienceAdelaideAustralia
  2. 2.Department of BiochemistryAlfred HospitalPrahranAustralia

Personalised recommendations