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Lactoferrin pp 183–196Cite as

Lactoferrin Gene Promoter in Human and Mouse

Analogous and Dissimilar Characteristics

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Part of the book series: Advances in, Experimental Medicine and Biology ((AEMB,volume 357))

Abstract

Lactoferrin promoter and the 5′-flanking region of both human and mouse were isolated from a genomic library constructed with lambda phage. A 2.0 kbp Sac I fragment of the human clone (HLF031a.30) and a 3.0 kbp Eco R I/Hinc II fragment of the mouse clone (mL14p9E) containing the lactoferrin promoter, 5′-flanking region, first exon and partial intron were sequenced completely. There were many sequence homologies between human and mouse at the promoter/enhancer (1 to -363) region, yet substantial divergence was observed beyond this region. To determine the promoter activity, 5′-deletion mutants of the mouse lactoferrin gene were linked to a CAT-reporter plasmid and transfected into the human endometrium carcinoma cell line, RL95-2. We identified a number of positive and negative regulatory sequences as well as the estrogen-response element in the 5′-flanking region of the lactoferrin gene. The imperfect estrogen response elements of both human and mouse are functional as demonstrated by transfection experiments, band-shift assay and DNase I footprint analysis. The molecular mechanism that governs the estrogen-stimulated response, however, differs between human and mouse.

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References

  • Arnold RR, Cole MF, McGhee JR. (1976) A bactericidal effect for human lactoferrin. Science 197: 263–265.

    Article  Google Scholar 

  • Birgens HS. (1991) The interaction of lactoferrin with human monocytes. Dan. Med. Bull. 38: 244–252.

    PubMed  CAS  Google Scholar 

  • Broxmeyer HE. (1992) Suppressor cytokines and regulation of myelopoiesis; biology and possible clinical uses. Am. J. Pediatr. Hemato. Oncol. 14:22–30

    Article  CAS  Google Scholar 

  • Dynan WS, Tjian R. (1983) The promoter-specific transcription factor Spl binds to upstream sequences in the SV40 early promoter. Cell 35: 79–87.

    Article  PubMed  CAS  Google Scholar 

  • Gasser SM, Laemmli UK. (1986) Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster. Cell 46: 521–530.

    Article  CAS  Google Scholar 

  • Goodman AS, Young LG. (1981) Immunological identification of lactoferrin as a shared antigen on radioiodinated human sperm surface and in radioiodinated human seminal plasma. J. Reprod. Immunol. 3: 99–108.

    Article  PubMed  CAS  Google Scholar 

  • Green MR, Pastewka JV. (1978) Lactoferrin is a marker for prolactin response in mouse mammary expiants. Endocrinology 103: 1510–1513.

    Article  CAS  Google Scholar 

  • Hekman A, Rumke P. (1969) The antigens of human seminal plasma (with special reference to lactoferrin as a spermatozoa-coating antigen). P549-552. In H. Peeters. (ed.) Protides Biological Fluids Colloquium. Vol.16. Pergamon Press Oxford.

    Google Scholar 

  • Johnston JJ, Rintels P, Chung J, Sather J, Benz EJ, Berliner N. (1992) Lactoferrin gene promoter: Structural integrity and nonexpression in HL60 cells. Blood 79: 2998–3006.

    PubMed  CAS  Google Scholar 

  • Kilewer SA, Umesono K, Heyman RA, Mangelsdorf DJ, Dyck JA, Evans RM. (1992) Retinoid X receptor-COUP-TF interactions modulate retinoic acid signaling. Proc. Natl. Acad. Sci. USA 89: 1448–1452.

    Article  Google Scholar 

  • Kilpatrick MW, Klysik J, Singleton CK, Zarling DA, Jovin TM, Hanau LH, Erlanger BF, Wells RD. (1984) Intervening sequences in human fetal globin genes adopt left-handed Z helices. J. Biol. Chem. 259: 7268–7274.

    PubMed  CAS  Google Scholar 

  • Liu YH, Teng CT (1991) Characterization of estrogen responsive lactoferrin gene promoter. J. Biol. Chem. 266: 21880–21885

    PubMed  CAS  Google Scholar 

  • Liu YH, Teng CT. (1992) Estrogen response module of the mouse lactoferrin gene contains overlapping chicken ovalbumin upstream promoter transcription factor and estrogen receptor-binding elements. Mol. Endocrinol. 6: 355–364.

    Article  PubMed  CAS  Google Scholar 

  • Liu YH, Yang N, Teng CT. (1993) COUP-TF acts as competitive repressor for estrogen-receptor mediated activation of the mouse lactoferrin gene. Mol. Cell. Biol. 13: 1836–1846.

    PubMed  CAS  Google Scholar 

  • Maniatis T, Goodbourn S, Fischer JA. (1987) Regulation of inducible and tissue-specific gene expression. Science 236: 1237–1245.

    Article  PubMed  CAS  Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J. (1982) Maxam Gilbert chemical degredation of DNA method, p. 13.11-13.13. In Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor N.Y.

    Google Scholar 

  • Masson PL, Heremans JF. (1971) Lactoferrin in milk from different species. Comp. Biochem. Physiol.-B. 39: 119–129.

    Article  PubMed  CAS  Google Scholar 

  • McKnight S, Tjian R. (1986) Transcriptional selectivity of viral genes in mammalian cells. Cell 46: 795–805.

    Article  PubMed  CAS  Google Scholar 

  • McMaster MT, Teng CT, Dey SK, Andrews GK. (1992) Lactoferrin in the mouse uterus: analyses of the preimplantation period and regulation by ovarian steroids. Mol. Endocrinol. 6: 101–111.

    Article  PubMed  CAS  Google Scholar 

  • Newbold RR, Teng CT, Beckman WC Jr., Jefferson WN, Hanson RB, Miller JV, McLachlan JA. (1992) Fluctuations of lactoferrin protein and mRNA in the reproductive tract of the mouse during the estrous cycle. Biol. Reprod. 47: 903–915.

    Article  PubMed  CAS  Google Scholar 

  • Panella TJ, Liu Yh, Huang AT, Teng CT. (1991) Polymorphism and altered methylation of the lactoferrin gene in normal leukocytes, leukemic cells and breast cancer. Cancer Res. 51: 3037–3043.

    PubMed  CAS  Google Scholar 

  • Pentecost BT, Teng CT. (1987) Lactotransferrin is the major estrogen inducible protein of mouse uterine secretions. J. Biol. Chem. 262: 10134–10139.

    PubMed  CAS  Google Scholar 

  • Rachman F, Casimiri V, Psychoyos A, Bernard O. (1983) Immunoglobulins in the mouse uterus during the oestrous cycle. J. Reprod. Fert. 69: 17–21.

    Article  CAS  Google Scholar 

  • Rado TA, Bollekens J, St Laurent G, Parker L, Benz EJ. (1984) Lactoferrin biosynthesis during granulocytopoiesis. Blood 64: 1103–1109.

    PubMed  CAS  Google Scholar 

  • Sagami I, Tsai SY, Wang H, Tsai MJ, O’Malley BW. (1986) Identification of two factors required for transcription of the ovalbumin gene. Mol. Cell. Biol. 6: 4259–4267.

    PubMed  CAS  Google Scholar 

  • Spitzner JR, Chung IK, Muller MT. (1990) Eukaryotic toposiomerase II preferentially cleaves alternating purine-pyrimidine repeats. Nucleic Acids Res. 18: 1–11.

    Article  PubMed  CAS  Google Scholar 

  • Teng CT, Liu YH, Yang NY, Walmer D, Panella T. (1992) Differential molecular mechanism of the estrogen action that regulates lactoferrin gene in human and mouse. Mol. Endocrinol. 6: 1969–1981.

    Article  PubMed  CAS  Google Scholar 

  • Teng CT, Pentecost BT, Chen YH, Newbold RR, Eddy EM, McLachlan JA. (1989) Lactoferrin gene expression in the mouse uterus and mammary gland. Endocrinology 124: 992–999.

    Article  PubMed  CAS  Google Scholar 

  • Teng CT, walker MP, Bhattacharyya SN, Klapper DG, DiAugustine RP, McLachlan JA. (1986) Purification and properties of an oestrogen-stimulated mouse uterine glycoprotein (approx. 70kDa). Biochem. J. 240: 413–422.

    PubMed  CAS  Google Scholar 

  • Tachi C., Tachi S, Knyszynski A, Linder HR. (1981) Possible involvement of macrophages in embryo-maternal relationships during embryo implantation in the rat. J. Exp. Zool. 217: 81–92.

    Article  PubMed  CAS  Google Scholar 

  • Tourville DR, Ogra SS, Lippes J, Tomasi Jr. TB. (1970) The human female reproductive tract: Immunohistological localization of γA, γG, γM secretory “piece,” and lactoferrin. Am. J. Obstet. Gynecol. 108: 1102–1108.

    PubMed  CAS  Google Scholar 

  • Tsai SF, Martin D, Zon LI, D’Andrea A, Wong G, Orkin S. (1989) Cloning of cDNA for the major DNA-bind-ing protein of the erythroid lineage through expression in mammalian cells. Nature 339: 446–451.

    Article  PubMed  CAS  Google Scholar 

  • Walmer DK, Wrona MA, Hughes CL, Nelson KG. (1992) Lactoferrin expression in the mouse reproductive tract during the natural estrous cycle: correlation with circulating estradiol and progesterone. Endocrinology 131: 1458–1466.

    Article  PubMed  CAS  Google Scholar 

  • Wells RD, Collier DA, Hanvey JC, Shimizu M., Wohlrab F. (1988) The chemistry and biology of unusual DNA structures adopted by oligopurine.oligopyrimidine sequences. FASEB J. 2: 2939–2949.

    PubMed  CAS  Google Scholar 

  • Wichmann L, Vaalasti A., Vaalasti T, Tuohimaa P. (1989) Localization of lactoferrin in the male reproductive tract. Internat. J. Andrology 12: 179–186.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, NIH Research Triangle Park, North Carolina, 27709, USA

    Christina T. Teng

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  1. Christina T. Teng
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Editor information

Editors and Affiliations

  1. University of California, Davis, Davis, California, USA

    T. William Hutchens  & Bo Lönnerdal  & 

  2. Massey University, Palmerston North, New Zealand

    Sylvia V. Rumball

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© 1994 Springer Science+Business Media New York

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Teng, C.T. (1994). Lactoferrin Gene Promoter in Human and Mouse. In: Hutchens, T.W., Rumball, S.V., Lönnerdal, B. (eds) Lactoferrin. Advances in, Experimental Medicine and Biology, vol 357. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2548-6_18

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  • DOI: https://doi.org/10.1007/978-1-4615-2548-6_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6087-2

  • Online ISBN: 978-1-4615-2548-6

  • eBook Packages: Springer Book Archive

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