Abstract
Obtaining the complete cDNA is of critical importance for gene structure and expression studies. cDNA clones derived from libraries frequently lack the full-length 5’end of the sense strand. The RACE (rapid amplification of cDNA ends) PCR technique first described by Frohman et al (1988) has proven to be a versatile and fast method for obtaining the complete 5’end of cDNAs.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Chomczynsky P and Sacchi N. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987, 162:156–159
Gong JH, Sprenger H, Hinder F, Bender A, Schmidt A, Horch S, Nain M, and Gemsa D. Influenza a virus infection of macrophages: enhanced tumor necrosis factor-a (TNF-a) gene expression and lipopolysaccharide-triggered TNF-a release. J Immunol 1991, 147:3507–3513
Klenk HD and Rott R. On the structure of the influenza virus envelope. Virology 1972, 47:579–588
Nain M, Hinder F, Gong JH, Schmidt A, Bender A, Sprenger H, and Gemsa D. Tumor necrosis-a production of influenza A virus-infected macrophages and potentiating effect of lipopolysaccharides. J Immunol 1990, 145:1921–1929
Ruano G, Pagliaro EM, Schwartz TR, Lamy K, Messina D, Gaensslen RE and Lee HC. Heat-soaked PCR: an efficient method for DNA amplification with application to forensic analysis. Biotechniques 1992, 13:266–274
Shen WH and Hohn B. DMSO improves PCR amplification of DNA with complex secondary structure. TIG 1992, 8:227
Smith KT, Long CM, Bowman B, and Manos M. Using cosolvents to enhance PCR amplification. Amplifications: A Forum for PCR Users 1990, 5:16–17
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media New York
About this chapter
Cite this chapter
Bacher, M., Hofmann, P., Gemsa, D. (1994). Resolution of a Secondary Structure in an Unknown mRNA 5’ End. In: Rolfs, A., Weber-Rolfs, I., Finckh, U. (eds) Methods in DNA Amplification. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2530-1_8
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2530-1_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-44908-6
Online ISBN: 978-1-4615-2530-1
eBook Packages: Springer Book Archive