Resolution of a Secondary Structure in an Unknown mRNA 5’ End
Obtaining the complete cDNA is of critical importance for gene structure and expression studies. cDNA clones derived from libraries frequently lack the full-length 5’end of the sense strand. The RACE (rapid amplification of cDNA ends) PCR technique first described by Frohman et al (1988) has proven to be a versatile and fast method for obtaining the complete 5’end of cDNAs.
KeywordsGlycerol DMSO Agarose Bromide Influenza
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