Abstract
Extraction of nucleic acids from samples for PCR is often time-consuming and may involve a large number of pipetting and transferring steps, with the addition of a number of reagents. This may cause loss of valuable target material and cross-contamination, and increase the opportunity for errors. It may also reduce the number of samples that might conveniently be handled at one time.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van-Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990, 28: 495–503
van den Brule AJ, Meijer CJ, Bakels V, Kenemans P, Walboomers JM. Rapid detection of human papillomavirus in cervical scrapes by combined general primer-mediated and type-specific polymerase chain reaction. J Clin Microbiol 1990, 28: 2739–2743
Buffone GJ. Improved amplification of cytomegalovirus DNA from urine after purification of DNA with glass beads. Clin Chem 1992, 38: 2360
Cheyrou A, Guyomarc’h C, Jasserand P, Blouin P. Improved detection of HBV DNA by PCR after microwave treatment of serum. Nucleic Acids Res 1991, 19: 4006.
Demeke T and Adams RP. The effect of plant polysaccharides and buffer additives on PCR. BioTechniques 1992, 12: 332–334
Grimprel E, Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniotic fluid, fetal and neonatal sera and cerebrospinal fluid. J Clin Microbiol 1991, 29: 1711–1718
Holodniy M, Kim S, Katzenstein D, Konrad M, Groves E, Merigan TC. Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol 1991, 29: 676–679
Khan G, Kangro HO, Coates PJ, Heath RB. Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA. J Clin Path 1991, 44: 360–365
Klapper PE, Cleator GM, Tan SV, Guiloff RJ, Scaravilli F, Ciardi M, Aurelius E, Forsgren M. Diagnosis of herpes simplex encephalitis with PCR. The Lancet 1993, 341:691
Picard C, Ponsormet C, Paget E, Nesme X, Simonet P. Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction. Appl Environ Microbiol 1992, 58: 2717–2722
Ruano G, Pagliaro EM, Schwartz TR, Lamy K, Messina D, Gaensslen RE, Lee HC. Heat-soaked PCR: an efficient method for DNA amplification with applications to forensic analysis. BioTechniques 1992, 13: 266–574
Sen S, Rahl S, Freireich EJ, Hewitt R, Stass SA. Detection of extrachromosomal circular DNA sequences from tumour cells by alkaline lysis, Alu-polymerase chain reaction technique. Mol Carcinog 1992, 5:107–110.
Singer-Sam J. The use of Chelex to improve the PCR signal from a small number of cells. Amplifications 1989, 3:11
Soini H, Skurnik M, Liippo K, Tala E, Viljanen MK. Detection and identification of mycobacteria by amplification of a segment of the gene coding for the 32-kilodalton protein. J Clin Microbiol 30: 2025–2028
Walsh PS, Metzger DA, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 1991, 10: 506–513
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media New York
About this chapter
Cite this chapter
Ochert, A., Slomka, M.J., Ellis, J., Teo, C.G. (1994). Use of Chelex 100TM in the Extraction of Viruses from Diverse Cell-Free Clinical Samples for PCR. In: Rolfs, A., Weber-Rolfs, I., Finckh, U. (eds) Methods in DNA Amplification. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2530-1_6
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2530-1_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-44908-6
Online ISBN: 978-1-4615-2530-1
eBook Packages: Springer Book Archive