Abstract
Genetic polymorphism of keratins at the protein level due to allelic variation has been described for Kl, K4, K5, and K10. In order to understand the molecular basis of the differences among the alleles of these genes, we have analyzed their N- and C-terminal domains following amplification of genomic DNA by the polymerase chain reaction. Whereas the Kl and the K10 alleles differ in size of their carboxyl-terminal V2 subdomains, the alleles of the K4 and K5 genes diverge in their amino-terminal domains.
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© 1994 Springer Science+Business Media New York
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Mischke, D., Wanner, R., Korge, B.P. (1994). Polymorphic Keratins as Detected by PCR and SSCP. In: Rolfs, A., Weber-Rolfs, I., Finckh, U. (eds) Methods in DNA Amplification. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2530-1_4
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DOI: https://doi.org/10.1007/978-1-4615-2530-1_4
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