Abstract
The polymerase chain reaction (PCR; Saiki et al 1988) is increasingly being used to quantify the number of copies of nucleic acid target in a sample. PCR quantification is important for many research and clinical applications such as analysis of gene expression (Gilliand et al 1990; Murphy et al 1990; Hoof et al 1991) or monitoring viral (Kellog et al 1990; Holodniy et. al 1991; Menzo et al 1992; Bavin et al 1993) and bacterial (Leigh et al 1993) infections. Due to the sensitivity of PCR, small variations in the reaction efficiency will result in significant differences in the amount of final product formed. A number of strategies have been developed which can minimize the variability of the reaction and hence increase the robustness of using PCR as a quantitative technique (Ferre 1992).
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© 1994 Springer Science+Business Media New York
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Cross, L., Potts, C., Anson, J.G. (1994). Sensitive and Rapid Detection and Quantification of Nucleic Acids. In: Rolfs, A., Weber-Rolfs, I., Finckh, U. (eds) Methods in DNA Amplification. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2530-1_3
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DOI: https://doi.org/10.1007/978-1-4615-2530-1_3
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