Abstract
Platelet-Endothelial Cell Adhesion Molecule (PECAM-1/CD31) is a member of the immunoglobulin gene superfamily 1. It consists of six extracellular C2-like immunoglobulin domains and reveals extensive homology with Carcino-Embryonic Antigen (CEA) and Intracellular Cell Adhesion Molecule-1 (ICAM-1)1. It is a type I transmembrane protein, carrying an 118aa cytoplasmic tail. PECAM-1 /CD31 is present not only on platelets and endothelia but also on monocytes, granulocytes and lymphocytes, predominantly of the naive phenotype1–7. It stabilizes cell-cell contacts between endothelial cells, activates high affinity states of in-tegrins on lymphocytes 3,7 ,and participates in the sequential adhesive events leading to arrest and extravasation of blood-borne cells into tissues 5. Here, we describe the distribution of PECAM-1/CD31 on different subsets of murine LAK cells using the monoclonal antibody EA-3. LAK cells, a heterogeneous mixture of IL-2 activated T- and NK-like cells, have been shown to reduce the number of metastases8–10 and to infiltrate into sites of lesions of established metastases 11, 12. On LAK cells, PECAM-1/CD31 has been shown to participate in the activation of high-affinity states of the integrin LFA-1 13. It is therefore conceivable that CD31+ LAK cells have advantages in infiltrating tumorous tissues.
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Newman, P. J., Berndt, M. C., Gorski, J., White, G. C., Lyman, S., Paddock, C. and Müller, W. A., PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily, Science 247:1219 (1990).
Stockinger, H., Gadd, S. J., Eher, R., Majdic, O., Schreiber, W., Kasinrerk, W., Strass, B., Schnabl, E. and Knapp, W., Molecular characterization and functional analysis of the leukocyte surface protein CD31, J. Immunol 145: 3889 (1990).
Albelda, S. M., Oliver, P. D., Romer, L. H. and Buck, C. A., EndoCAM: A Novel Endothelial Cell-Cell Adhesion Molecule, J. Cell Biol. 110:1227 (1990).
Torimoto, Y., Rothstein, D. M., Dang, N. H., Schlossman, S. F. and Morimoto, C., CD31, a novel cell surface marker for CD4 cells of suppressor lineage, unaltered by state of activation, J. Immunol 148:388 (1992).
Tanaka, Y., Albelda, S. M., Horgan, K. J., van Seventer, G., Shimizu, Y., Newman, W., Hallam, J., Newman, P. J., Buck, C. A. and Shaw, S., CD31 expressed on distinctive T cell subsets is a preferential amplifier of βl integrin-mediated adhesion, J Exp Med 176:245 (1992).
Cabañas, C, Sanchez-Madrid, F., Bellon, T., Figdor, C. G., Te Velde, M., Fernandez, M., Acevedo, A. and Barnabeu, C, Characterization of a novel myeloid antigen regulated during differentiation of monocytic cells, Eur. J. Immunol. 19:1373 (1989).
Albelda, S. M., Muller, W. A., Buck, C. A. and Newman, P. J., Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule, J Cell Biol 114:1059 (1991).
Mazumder, A. and Rosenberg, S. A., Successful Immunotherapy of Natural Killer-Resistant Established Pulmonary Melanoma Métastases by the Intravenous Adoptive Transfer of Syngeneic Lymphocytes Activated in vitro by Interleukin-2, J. Exp. Med. 159:495 (1984).
Rosenberg, S. A., Lotze, M. T., Muul, L. M., Chang, A. E., Avis, F. P., Leitman, S., Linehan, W. M., Robertson, C. N., Lee, R. E., Rubin, J. T., Seipp, C. A., Simpson, C. G. and White, D. E., A Progress Report on the Treatment of 157 Patients with advanced Cancer using Lymphokine-Activated Killer Cells and Interleukin-2 or high-dose Interlukin-2 alone, N. Engl. J. Med. 316: 889 (1987).
Schwarz, R. E., Vujanovic, N. L. and Hiserodt, J. C, Enhanced Antimetastatic Activity of Lymphokine-activated Killer Cells Purified and Expanded by Their Adherence to Plastic, Cancer Res. 49:1441 (1989).
Basse, P. H., Nannmark, U., Johansson, B. R., Herberman, R. B. and Goldfarb, R. H., Establishment of Cell-to-Cell Contact by Adoptively Transferred Adherent Lymphokine-Activated Killer Cells With Metastatic Murine Melanoma Cells, J. Natl. Cancer Inst. 83:944 (1991).
Basse, P., Herberman, R. B., Nannmark, U., Johansson, B. R., Hokland, M., Wasserman, K. and Goldfarb, R. H., Accumulation of Adoptively Transferred Adherent, Lymphokine-activated Killer Cells in Murine Metastases, J. Exp. Med. 174:479 (1991).
Piali, L., Albelda, S. M., Baldwin, H.S., Hammel, P., Gisler, R.H. and Imhof, B. A., Murine Platelet Endothelial Cell Adhesion Molecule (PECAM-D/CD31 modulates β2 Integrins on Lymphokine-Activated Killer Cells, Eur. J. Immunol. in press: (1993).
Gunji, Y., Vujanovic, N. L., Hiserodt, J. C, Herberman, R. B. and Gorelik, E., Generation and Characterization of Purified Adherent Lymphokine-Activated Killer Cells in Mice, J. Immunol. 142:1748 (1989).
Karasuyama, H., Kudo, A. and Melchers, F., The Proteins Encoded by the VpreB and λ5 Pre-B Cell-specific Genes Can Associate with Each Other and with μ Heavy Chain, J. Exp. Med. 172:969 (1990).
Herberman, R. B., Lymphokine-activated killer cell activity, Immunol. Today 8:178 (1987).
Yang, J. C, Mulé, J. J. and Rosenberg, S. A., Murine Lymphokine-Activated Killer (LAK) Cells; Phenotypic characterization of the Precursor and Effector cells, J.Immunol. 137:715(1986).
Seaman, W. E., Sleisenger, M., Eriksson, E. and Koo, G. C, Depletion of Natural Killer Cells in Mice by Monoclonal Antibody to NK-1.1, J. Immunol. 138: 4539 (1987).
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Piali, L., Imhof, B.A., Gisler, R.H. (1994). Adhesion Molecule Pecam-1/CD31 is Expressed on Defined Subsets of Murine Lak Cells. In: Heinen, E., Defresne, M.P., Boniver, J., Geenen, V. (eds) In Vivo Immunology. Advances in Experimental Medicine and Biology, vol 355. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2492-2_25
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DOI: https://doi.org/10.1007/978-1-4615-2492-2_25
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