Abstract
The initiation of thymus-dependent (TD) antibody responses requires class ll-restricted antigen-specific helper T cell cognate interactions with B cells. None of the molecularly cloned cytokines, alone or in combination, can replace the contact-dependent requirement for B cell activation1. The molecule which mediates the contact-dependent signal is a 39 kD membrane protein which is expressed on the surface of activated T-helper (Th) cells2,3. This membrane protein, gp39, was identified as the ligand for the B cell membrane protein CD40. CD40, a mitogenic receptor expressed on all mature B lymphocytes4, is a type I membrane protein and member of the TNF receptor family5. Evidence that CD40 is an important receptor on B cells is derived from studies that anti-CD40 mAb and cofactors such as anti-immunoglobulin (Ig) and cytokines initiate both B cell growth and differentiation6,7.
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References
D.C. Parker. T cell dependent B cell activation, Annu. Rev. Immunol. 11:331 (1993).
R.J. Armitage, W.C. Fanslow, L Strockbine, T.A. Sato, K.N. Clifford, B.M. Macduff, D.M. Anderson, S.D. Gimpel.T. Davis-Smith, C.R. Maliszewski, E.A. Clark, C.A. Smith, K.H. Grabstein, D. Cosman, and M.K. Spriggs. Molecular and biological characterization of a murine ligand for CD40, Nature 257:80 (1992).
R.J. Noelle,, M. Roy, D.M. Shepherd, I. Stamenkovic, J.A. Ledbetter, and A.A. Aruffo. A novel ligand on activated helper T cells bind CD40 and transduces the cognate activation of B cell, Proc. Natl. Acad. Sci. USA 89:6550 (1992).
E.A. Clark, and P.J. Lane. Regulation of human B-cell activation and adhesion, Ann. Rev. Immunol. 9:97(1991).
I. Stamenkovic, E.A. Clark, and B. Seed. A B lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinoma, EMBO J. 8:1403 (1989)
H.H. Jabara, S.M. Fu, R.S. Geha, and D. Vercelli. CD40 and lgE:Synergism between anti-CD40 monoclonal antibody and interleukin 4 in the induction of IgE synthesis by highly purified human B cells, J. Exp. Med. 172:1861 (1990).
J. Banchereau, P. De Paoli, A. Vallé, E. Garcia, and F. Rousset. Long-term human B cell lines dependent on interleukin-4 antibody to CD40, Science 251:70 (1991).
D. Hollenbaugh, Grosmaire, L.S., Kullas, C.D., Chalupny, N.J., Braesh-Andersen, Noelle, R.J., Stamenkovic, I., Ledbetter, J.A, and A. Aruffo. The human T cell antigen gp39, a member of the TNF gene family, is aligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity, EMBO 11:4313 (1992).
T. Farah, and C. A. Smith. Emerging cytokine family, Nature 358:26 (1992).
R. J. Noelle, J. Daum, W.C. Bartlett, J. McCann, and D.M. Shepherd. Cognate interactions between helper T cells and B cells; V. reconstitution of T helper cell function using purified plasma membranes from activated Th1 and Th2 T helper cells and lymphokines, J. Immunol. 146:1118(1991).
K.H. Grabstein, Maliszewski, K. Shanebeck, T.A. Sato, M.K. Spriggs, W.C. Fanslow, and R.J. Armitage. The regulation of T cell-dependent antibody formation in vitro by CD40 ligand and IL-2, J. Immunol. 150:3141 (1993).
R.J. Armitage, B.M. Macduff, M.K. Spriggs, and W.C. Fanslow. Human B cell proliferation and Ig secretion induced by recombinant CD40 ligand are modulated by soluble cytokines, J. Immunol. 150:3671 (1993).
A.J.M Van den Eertwegh, R.J. Noelle, M. Roy, D.M. Shepherd, A. Aruffo, J. A. Ledbetter, W.J.A. Boersma and E. Claassen. In vivo CD40-gp39 interactions are essential for thymus dependent humoral immunity. I. In vivo expression of CD40 ligand, cytokines and antibody production delineates sites of cognate T-B cell interactions, J. Exp. Med. (in press).
T.M. Foy, Durie, F.H., Shepherd, D.M., Aruffo, A., Ledbetter, J.A, and R.J. Noelle. In vivo CD40-gp39 interactions are essential for thymus dependent humoral immunity. II. Prolonged suppression of the humoral immune response by an antibody to the ligand for CD40, gp39, J. Exp. Med. (in press).
Y. Liu, G.D. Johnson, J. Gordon, and CM. Maclennan. Germinal centres in T-cell dependent antibody responses, Immunol. Today 13:17 (1992).
E.B. Jacobson, L.H. Caporale, and G.J. Thorbecke. Effect of thymus cell injections on germinal center formation in lymphoid tissues of nudes (thymusless) mice, Cell. Immunol. 13:416 (1974).
Y.L Liu, D.E. Joshua, G.T. Williams, C.A. Smith, J. Gordon and I.C.M. MacLennan. 1989. Mechanism of antigen-driven selection in germinal centres, Nature 342:929 (1989).
E. Claassen, N. Kors, and N. Van Rooyen. Influence of carriers on the development and localization of anti-trinitrophenyl antibody forming cells in the murine spleen, Eur. J. Immunol. 16:271 (1986).
T.A. Springer, A. Bhattacharya, J.T. Cardoza, F. Sanchez-Madrid. Monoclonal antibodies specific for rat lgG1, lgG2a and lgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies, Hybrid. 1:257 (1982).
E. Claassen, and L Adler. Sequential double immunocytochemical staining for the in situ identification of an auto-anti-allotype immune response in allotype suppressed rabbits, J. Histochem. Cytochem. 36:1455 (1988).
A.J.M. Van den Eertwegh, J.D. Laman, M.M. Schellekens, W.J.A. Boersma, and E. Claassen. Complement mediated follicular localization of T-independent type 2 antigens: the role of marginal zone macrophages revisited, Eur. J. Immunol. 22:719 (1992).
A.J.M. Van den Eertwegh, M.J. Fasbender, M.M. Schellekens, A. Van Oudenaren, W.J.A. Boersma, and E. Claassen. In vivo kinetics and characterization of IFN-γ-producing cells during a thymus independent immune response, J. of Immunol. 147:439 (1991).
E. Claassen, N. Kors, and N. Van Rooijen. Immunomodulation with liposomes: The immune response elicited by liposomes with entrapped dichloromethylenediphosphonate and surface associated antigen or hapten, Immunology 60: 509 (1987).
Q. Vos, E. Claassen, and R. Benner. Substantially increased sensitivity of the spot-ELISA for the detection of anti-insulin antibody-secreting cells during a capture and enzyme-conjugated insulin, J.lmmunol. Meth. 126:89 (1990).
G. Koch, and R. Benner. Differential requirement for B memory and T memory cells in adoptive antibody formation in mouse bone marrow, Immunol. 45:697 (1982).
J.J. Mond, J. Farrar, W.E. Paul, J. Fuller-Farrar, M. Schaefer, and M. Howard. 1983.T cell dependence and factor reconstitution of in vitro antibody responses to TNP-B. abortus and TNP-Ficoll: restoration of depleted responses with chromatographed fractions of a T-cell derived factor, J. Immunol. 131:633 (1983).
R. Endres, E. Kushnir, J.W. Kappler, P. Marrack, and S.C. Kinsky. A requirement for T cell help factors in antibody responses to“ T-independent” antigens, J. Immunol. 130:781 (1983).
D. Gray. Recruitment of virgin B cells into an immune response is resticted to activation outside follicles, Immunol 65:659 (1988).
R.H. Vonderheide and S.V. Hunt. Immigration of thoracic duct B lymphocytes into established germinal centers in the rat, Eur. J. Immunol. 20:79 (1990).
A.J.M. Van den Eertwegh, W.J.A. Boersma, and E. Claassen. Immunological functions and in vivo cell-cell interactions of T-lymphocytes in the spleen, Crit. Rev. Immunol. 11:337 (1992).
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van den Eertwegh, A.J.M., Vanneurs, M., Foy, T.M., Noelle, R.J., Boersma, W.J.A., Claassen, E. (1994). In Vivo GP39-CD40 Interactions Occur in the Non-Follicular Compartments of the Spleen and are Essential for Thymus Dependent Antibody Responses and Germinal Center Formation. In: Heinen, E., Defresne, M.P., Boniver, J., Geenen, V. (eds) In Vivo Immunology. Advances in Experimental Medicine and Biology, vol 355. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2492-2_13
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DOI: https://doi.org/10.1007/978-1-4615-2492-2_13
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