Identification of Mollicutes by Immunoblotting
Immunoblotting (IB) technique combines the resolution of gel electrophoresis with the specificity of immunochemical detection. It is used to detect specific antigens in a protein mixture with an antibody and to evaluate the purity or the complexity of this mixture. Since Towbin described the procedure (1979), numerous protocols evolved during the years using various gel systems, different immobilizing matrices, modified buffer systems, diversity of ligands and variety of washing and blocking conditions. There is no ideal procedure, and the technique should be examined according to one’s need and the conditions be determined empirically for each specific system investigated.
KeywordsAntigenic Variation Mycoplasma Pneumoniae Transfer Buffer Ureaplasma Urealyticum Transfer Apparatus
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