Identification of Mollicutes by Immunoblotting

  • Shulamith Horowitz
Part of the Federation of European Microbilogical Societies Symposium Series book series (FEMS, volume 62)

Abstract

Immunoblotting (IB) technique combines the resolution of gel electrophoresis with the specificity of immunochemical detection. It is used to detect specific antigens in a protein mixture with an antibody and to evaluate the purity or the complexity of this mixture. Since Towbin described the procedure (1979), numerous protocols evolved during the years using various gel systems, different immobilizing matrices, modified buffer systems, diversity of ligands and variety of washing and blocking conditions. There is no ideal procedure, and the technique should be examined according to one’s need and the conditions be determined empirically for each specific system investigated.

Keywords

Glycerol Urea Amide Pneumonia Glycine 

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Shulamith Horowitz
    • 1
  1. 1.Department of Microbiology and Immunology Faculty of Health SciencesBen-Gurion University of the NegevBeer-ShevaIsrael

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