In Vivo Studies of Ethanol Interactions with N-Methyl-D-Aspartate Systems in Rat Brain

  • Lynn C. Roper
  • Rueben A. Gonzales


Several electrophysiological and biochemical studies have indicated that N-methyl-D-aspartate (NMDA) receptor functions may be altered by ethanol (Gonzales, 1990). Many have suggested that ethanol potently and selectively inhibits the actions of agonists at the NMDA receptor (Hoffman et al., 1989; Lovinger et al., 1989; Gothert and Fink, 1989; Hoffman et al., 1990; Woodward and Gonzales, 1990). Others have implicated the involvement of the NMDA receptor in acute behavioral effects of ethanol such as memory dysfunction (Hoffman et al., 1990) and in certain aspects of ethanol withdrawal symptoms, such as seizures (Grant et al., 1990).


NMDA Receptor Striatal Slice Ethanol Inhibition Striatal Synaptosome Competitive NMDA Antagonist 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Benveniste, H., 1989, Brain microdialysis, J. Neurochem. 52: 1667–1679.PubMedCrossRefGoogle Scholar
  2. Birch, P.J., Grossman, C.J. and Hayes, A.G., 1988, 6, 7-Dinitro-quinoxaline-2,3-dione and 6-nitro-7-cyano-quinoxaline-2,3-dione antagonize responses to NMDA in the rat spinal cord via an action at the strychnine-insensitive glycine receptor, Eur. J. Pharmacol. 156: 177–180.PubMedCrossRefGoogle Scholar
  3. Carter, C.J., L’ Heureux, R., and Scatton, B., 1988, Differential control by N-methyl-D-aspartate and kainate of striatal dopamine release in vivo: a trans-striatal dialysis study. J. Neurochem, 51: 462–468.PubMedCrossRefGoogle Scholar
  4. Clow, D.W., and Jhamandas K., 1989, Characterization of L-glutamate action on the release of endogenous dopamine from the rat caudate-putamen, J. Pharmacol. Exp. Ther. 248: 722–728.PubMedGoogle Scholar
  5. Di Chiara, G., 1990, In vivo brain dialysis of neurotransmitters, TiPS. 11: 116–121.PubMedGoogle Scholar
  6. Gonzales, R.A., 1990, NMDA receptors excite alcohol research, TiPS. 11: 137–139.PubMedGoogle Scholar
  7. Gothert, M. and Fink, K., 1989, Inhibition of N-methyl-D-aspartate (NMDA)- and L-glutamate-induced noradrenaline and acetylcholine release in the rat brain by ethanol, Naunyn-Schmied. Arch Pharmacol., 340: 516–521.CrossRefGoogle Scholar
  8. Grant, K.A., Valverius, P., Hudspith, M., and Tabakoff, B., 1990, Ethanol withdrawal seizures and the NMDA receptor complex, Eur. J. Pharmacol. 176: 289–296.PubMedCrossRefGoogle Scholar
  9. Gysling K., Bustos, G., Concha, I., and Martinez, G., 1975, Effect of ethanol on dopamine synthesis and release from rat corpus striatum, Biochem. Pharmacol. 25: 157–162.CrossRefGoogle Scholar
  10. Hoffman, P.L., Rabe, C.S., Moses, F., and Tabakoff, B., 1989H, N-methyl-D-aspartate receptors and ethanol: inhibition of calcium flux and cyclic GMP production, J. Neurochem. 52: 1937–1940.Google Scholar
  11. Hoffman, P.L., Moses, F., and Tabakoff, B., 1989b, Selective inhibition by ethanol of glutamate-stimulated cyclic GMP production in primary cultures of cerebellar granule cells, Neuropharmacology 28: 1239–1243.PubMedCrossRefGoogle Scholar
  12. Hoffman, P.L., Rabe, C.S., Grant K.A., Valverius, P., Hudspith, M., and Tabakoff, B., 1990, Ethanol and the NMDA receptor, Alcohol. 7: 229–231.PubMedCrossRefGoogle Scholar
  13. Imperato, A., and Di Chiara G., 1986, Preferential stimulation of dopamine release in the nucleus accumbens of freely moving rats by ethanol, J. Pharmacol. Exp. Ther. 239: 219–228.PubMedGoogle Scholar
  14. Imperato, A., Honore, T., and Jensen, L.H., 1990, Dopamine release in the nucleus caudatus and in the nucleus accumbens is under glutamatergic control through non-NMDA receptors: a study in freely-moving rats, Brain Research 530: 223–228.PubMedCrossRefGoogle Scholar
  15. Keefe, K.A., Zigmond, M.J., and Abercrombie, E.D., 1990, Excitatory amino acid receptor involvement in the regulation of striatal extracellular dopamine, Ann. N.Y. Acad. Sci. 604: 614–615.CrossRefGoogle Scholar
  16. Khatib, S.A., Murphy, J.M. and McBride W.J., 1988, Biochemical evidence for activation of specific monoamine pathways by ethanol, Alcohol. 5: 295–299.PubMedCrossRefGoogle Scholar
  17. Lovinger, D.M., White, G., and Weight, F.F., 1989, Ethanol inhibits NMDA-activated ion current in hippocampal neurons, Science 243: 1721–1724.PubMedCrossRefGoogle Scholar
  18. Mayer, M.L. and Westbrook, G.L., 1987, The physiology of excitatory amino acids in the vertebrate central nervous system, Prog. Neurobiol. 28: 197–276.PubMedCrossRefGoogle Scholar
  19. Moghaddam, B., Gruen, R.J., Roth, R.H., Bunney, B.S., and Adams, R.N., 1990, Effect of L-glutamate on the release of striatal dopamine: in vivo dialysis and electrochemical studies, Brain Research. 518: 55–60.PubMedCrossRefGoogle Scholar
  20. Nowak, L., Bregestovski, P., Ascher, P., Herbet, A., Prochiantz, A., 1984, Magnesium gates glutamate-activated channels in mouse central neurons, Nature 309: 261–263.CrossRefGoogle Scholar
  21. Patel, J., Zinkard, W.C., Klika, A.B., Mangano, T.J., Keith, R.A., and Salama, A.I., 1990, 6, 7-Dintroquinoxaline-2,3-dione blocks the cytotoxicity of N-methyl-D-aspartate and kainate, but not quisqualate, in cortical cultures, J. Neurochem. 55: 114–121.Google Scholar
  22. Paxinos, G., and Watson, C., 1982, The Rat Brain in Stereotaxic coordinates, Academic Press, New York.Google Scholar
  23. Wang, J., 1991, Presynaptic glutamate receptors modulate dopamine release from striatal synaptosomes, J. Neurochem. 57: 819–822.PubMedCrossRefGoogle Scholar
  24. Woodward J.J. and Gonzales, R.A. Ethanol inhibition of N-methyl-D-aspartate-stimulated endogenous dopamine release from rat striatal slices: reversal by glycine, J. Neurochem. 54: 712–715.Google Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Lynn C. Roper
    • 1
  • Rueben A. Gonzales
    • 1
  1. 1.Department of PharmacologyUniversity of TexasAustinUSA

Personalised recommendations