Measurement of PO2 in a Murine Tumour and Its Correlation with Hypoxic Fraction
Radiation resistant hypoxic cells, found to exist in most animal and human solid tumours (Moulder and Rockwell,1984; Vaupel et al.,1989),are now believed to compromise the success of clinical radiotherapy (Dische,1989; Overgaard,1989).Numerous attempts have been made to try and identify those human tumours which contain hypoxic regions and therefore are most likely to benefit from therapies which can overcome hypoxia (for review see Horsman,1993).These techniques have included measurements of tumour vascularization, cryospectrophotometric estimates of intercapillary haemoglobin oxygen saturations, tumour metabolic activity, the binding of radioactive or fluorescently labelled nitroimidazole compounds and the determination of oxygen partial pressure (pO2) distributions using microelectrodes.
KeywordsRubber Carbon Monoxide Nicotinamide Fenton Hydralazine
Unable to display preview. Download preview PDF.
- Dische, S,. 1989, The clinical consequences of the oxygen effect, in: “The Biological Basis of Radiotherapy,” G.G. Steel, G.E. Adams, and A. Horwich, eds., Elsevier Science Publishers, Amsterdam, The Netherlands.Google Scholar
- Horsman, M.R., 1993, Hypoxia in tumours: its relevance, identification and modification, in:Current Topics in Clinical Radiobiology of Tumours, H.P. Beck-Bornholt, ed., Springer Verlag, Heidelberg (in press).Google Scholar
- Horsman, M.R., Kristjansen, P.E.G., Mizuno, M., Christensen, K., Chaplin, D.J., Quistorff, B., and Overgaard, J., 1992, Biochemical and physiological changes induced by nicotinamide in a C3H mouse mammary carcinoma and CDF1 mice, Int. J. Radiat. Oncol. Biol. Phys. 22:451.PubMedCrossRefGoogle Scholar
- Höckel, M., Knoop, C., Schlenger, K., Vorndran, B., Mitz, M., Knapstein, P.G., and Vaupel, P., 1993, Intratumoral pO2 predicts survival in advanced cancer of the uterine cervix, Radiother.Oncol. (In Press)Google Scholar
- Kolstad, P., 1968, Intercapillary distance, oxygen tension and local recurrence in cervix cancer, Scand.J. Clin.Lab. Invest. 106:145.Google Scholar
- Vaupel, P., 1979, Oxygen supply to malignant tumors, in: “Tumor Blood Circulation: Angiogenesis, Vascular Morphology and Blood Flow of Experimental and Human Tumors,” H.I. Peterson, ed., CRC Press Inc., Boca Raton, Florida.Google Scholar