Skip to main content
SpringerLink
Log in

Function of Isolated Rabbit Hearts Perfused with Erythrocyte Suspension is not Stable but Improvement may be Feasible with Hemoglobin Solution

  • Chapter
Oxygen Transport to Tissue XV

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 345))

Abstract

In order to study cardiac function many investigators use isolated heart preparations perfused with saline solutions. In isolated rabbit heart perfused with a Tyrode solution at 37 °C it has been shown that oxygen supply is partially limiting oxygen consumption1. Experiments with isolated Tyrode perfused rabbit hearts at 28 °C proved that oxygen supply was not limiting. This finding has been explained by increased oxygen solubility and decreased metabolic demand at the lower temperature2.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. J.H.G.M. van Beek, P. Bouma, and N. Westerhof. Oxygen uptake in saline-perfused rabbit heart is decreased to a similar extent during reductions in flow and in arterial oxygen concentration. Pflügers Arch. 414:82 (1989).

    Article  PubMed  Google Scholar 

  2. J.B. Hak, J.H.G.M. van Beek, M.H. van Wijhe, and N. Westerhof. Influence of temperature on the response time of mitochondria) oxygen consumption in isolated rabbit heart. J. Physiol. (Lond) 447:17 (1992).

    CAS  Google Scholar 

  3. C.S. Apstein, R.C. Dennis, L. Briggs, W.M. Vogel, J. Frazer and C.R. Valeri. Effect of erythrocyte storage and oxyhemoglobin affinity changes on cardiac function. Am. J. Physiol. 248:H508 (1985).

    PubMed  CAS  Google Scholar 

  4. J.F. Baron, E. Vicaut, X. Hou and M. Duvelleroy. Independent role of arterial O2 tension in local control of coronary blood flow. Am. J. Physiol. 258:H1388 (1990).

    PubMed  CAS  Google Scholar 

  5. S.R. Bergmann, R.E. Clark and B.E. Sobel. An improved isolated heart preparation for external assessment of myocardial metabolism. Am. J. Physiol. 236:H644 (1979).

    PubMed  CAS  Google Scholar 

  6. J.A. Leppo and D.J. Meerdink. Comparison of the myocardial uptake of a technetium-labelled isonitrile analogue and thallium. Circ. Res. 65:632 (1989).

    Article  PubMed  CAS  Google Scholar 

  7. Y. Goto, B.K. Slinker, and M.M. LeWinter. Similar normalized Emax and O2 consumption-pressurevolume area relation in rabbit and dog. Am. J. Physiol. 255:H366 (1988).

    PubMed  CAS  Google Scholar 

  8. K. Nishiki, M. Erecinska, and D.F. Wilson. Energy relationships between cytosolic metabolism and mitochondrial respiration in rat heart. Am. J. Physiol. 234:C73 (1978).

    PubMed  CAS  Google Scholar 

  9. P.T.M. Biessels, J.B. Hak, W.K. Bleeker, J.H.G.M. van Beek, and J.C. Bakker. Effects of modified hemoglobin solutions on the isolated rabbit heart. Biomaterials, Artificial Cells and Immobilization technology, in press.

    Google Scholar 

  10. J. van der Plas, A. de Vries-van Rossen, J.J. Koorevaar, A. Buursma, W.G. Zijlstra and J.C. Bakker. Purification and physical characteristics of a hemoglobin solution modified by coupling to 2-nor-2formylpyridoxal 5’-phosphate (NFPLP). Transfusion 28:525 (1988).

    Article  PubMed  Google Scholar 

  11. G.A.M. Berbers, W.K. Bleeker, P. Stekkinger, J. Agterberg, G. Rigter, and J.C. Bakker. Biophysical characteristics of hemoglobin intramolecularly cross-linked and polymerized. J. Lab. Clin. Med. 117:157 (1991).

    PubMed  CAS  Google Scholar 

  12. R.W. Baer, G.J. Vlahakes, P.N. Uhlig, and J.I.E. Hoffman. Maximum myocardial oxygen transport during anemia and polycythemia in dogs. Am. J. Physiol. 252:H1086 (1987).

    PubMed  CAS  Google Scholar 

  13. L.E.H. Whitby, and C.J.C. Britton. Disorders of the blood: Diagnosis, Pathology, Treatment, Technique. Ninth edition, Churchill, London (1963).

    Google Scholar 

  14. W.J. Williams, E. Beutler, A.J. Erslev and M.A. Lichtman. Hematology. Third Edition, McGraw-Hill, New York, USA (1983).

    Google Scholar 

  15. P.H. Huisman and J.J. Schipperheyn. The isolated heart-lung preparation. Martinus Nijhoff Medical Division. The Hague (1978).

    Book  Google Scholar 

  16. L.D. Segel, J.L. Ensunsa and W.A. Boyle III. Prolonged support of working rabbit hearts using fluosol-43 or erythrocyte media. Am. J. Physiol. 252:H349 (1987).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory for Physiology, Free University, 1081, BT, Amsterdam

    J. B. Hak, J. H. G. M. van Beek & N. Westerhof

  2. Central Laboratory of The Netherlands Red, Cross Blood Transfusion Service, 1006, AK, Amsterdam, The Netherlands

    P. T. M. Biessels & J. C. Bakker

Authors
  1. J. B. Hak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. T. M. Biessels
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. H. G. M. van Beek
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. C. Bakker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. Westerhof
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Johannes Gutenberg-Universität, Mainz, Germany

    Peter Vaupel  & Rolf Zander  & 

  2. University of Maryland Baltimore County, Baltimore, Maryland, USA

    Duane F. Bruley

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer Science+Business Media New York

About this chapter

Cite this chapter

Hak, J.B., Biessels, P.T.M., van Beek, J.H.G.M., Bakker, J.C., Westerhof, N. (1994). Function of Isolated Rabbit Hearts Perfused with Erythrocyte Suspension is not Stable but Improvement may be Feasible with Hemoglobin Solution. In: Vaupel, P., Zander, R., Bruley, D.F. (eds) Oxygen Transport to Tissue XV. Advances in Experimental Medicine and Biology, vol 345. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2468-7_39

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-2468-7_39

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6051-3

  • Online ISBN: 978-1-4615-2468-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation