Method for Simultaneous Determination of the Distribution of Capillary Plasma Flow and Myocyte Redox State (NADH-Fluorescence) in the Hypoperfused Myocardium of the Anesthetized Rat
Narrowing of a coronary artery does not induce ischemia to the same degree throughout the affected myocardium but leads to significant spatial differences in the depression of local supply conditions (e.g. Jennings et al, 1975). One indication for the development of such a regional heterogeneity in tissue supply on even a rather low scale comes from topographic analyses of the blue NADH-fluorescence on the surface of isolated hearts perfused under ischemic conditions (Steenbergen et al, 1977). While it can be safely assumed that this blue fluorescence indicates extreme degrees of oxygen deficiency in the myocytes due to accumulation of mitochondrial NADH, the question whether this signal is indicative for a loss of local blood flow as well, could only insufficiently be answered until now.
KeywordsIschemic Condition Coronary Perfusion Pressure Local Blood Flow Mitochondrial NADH Hypoperfused Myocardium
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- Steenbergen, C., and Williamson, J.R., 1980, Heterogeneous coronary perfusion during myocardial hypoxia. Adv. in Myocardiology, Vol. 2, eds. M.v. Tajuddin, B. Bhatia, H.H. Siddiqui, G. Rona, University Park Press, Baltimore, pp 271–284.Google Scholar