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The Antibody Approach of Labeling Blood Cells

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Radiolabeled Blood Elements

Part of the book series: NATO ASI Series ((NSSA,volume 262))

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Abstract

Although the science of blood cell labeling using monoclonal antibodies directed against specific cellular antigens is still in its early stages, considerable progress has recently been accomplished in this area. The monoclonal antibody approach offers the promise of greater selectivity and enhanced convenience since specific cell types can be labeled in vivo, thus eliminating the need for complex and damaging cell separation procedures. This article focuses on these developments with primary emphasis on antibody labeling of platelets and leukocytes. The advantages and the shortcomings of the recently reported techniques are critically assessed and evaluated.

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References

  • Andres RY, Seybold K, Tiefenauer L, et al (1988) Radioimmunoscintigraphic localization of inflammatory lesions: Concept, radiolabeling and in vitro testing of a granulocyte antibody. Eur J Nucl Med, 13:582

    Article  PubMed  CAS  Google Scholar 

  • Baum RP, Hentel A, Bosslet K, et al (1990) First use of Tc-99m labeled monoclonal antibody directed against the T cell receptor for immunoscintigraphic detection of transplant rejection. Eur J Nucl Med, 16:163 (abstr)

    Google Scholar 

  • Becker W (1990) Immunoscintigraphy of infectious lesions. In: Immunoscintigraphy: Facts and Fictions, edited by D.L. Munz and D. Emrich, Elsevier, Amsterdam, pp. 159–171

    Google Scholar 

  • Becker W, Borst U, Fischbach W, et al (1989) Kinetic data of in-vivo-labelled granulocytes in humans with a murine Tc-99m-labelled monoclonal antibody. Eur J Nucl Med, 15: 361

    Article  PubMed  CAS  Google Scholar 

  • Becker W, Horneff G, Emmrich F, et al (1990a) Tc-99m labeled anti CD-4 helper (lymphocytes) antibody scans for imaging of rheumatoid arthritis. Eur J Nucl Med, 16:401 (abstr)

    Google Scholar 

  • Becker W, Saptogino A, Wolf F (1991) Diagnostic accuracy of a late single Tc-99m-granulocyte antibody scan in inflammatory or infectious diseases. J. Nucl Med, 32: 1002 (abst)

    Google Scholar 

  • Berberich R, Sutter M, Oberhausen E (1991) Inflammation detection with Tc-99m labeled monoclonal antibody for granulocytes. Dialogos (Siemens), 3/91: pp. 6–11

    Google Scholar 

  • Bosslet K, Lüben G, Schwarz A, et al (1985) Immunohistochemical localization and molecular characteristics of three monoclonal antibody-defined epitopes detectable on carcinoembryonic antigen (CEA). Int J Cancer, 36:75

    Article  PubMed  CAS  Google Scholar 

  • Buchegger F, Schreyer M, Carrel S, et al (1984) Monoclonal antibodies identify a CEA crossreacting antigen of 95 kD (NCA-95) distinct in antigenicity and tissue distribution from the previously described NCA of 55 kD. Int J Cancer, 33: 643

    Article  PubMed  CAS  Google Scholar 

  • Chouraqui P, Davidson M, Thomas C, et al (1991) Effect of thrombus age on uptake of Tc-99m labeled anti-activated platelet monoclonal antibody. J Nucl Med, 32:1013 (abstr)

    Google Scholar 

  • Chouraqui P, Maddahi J, Fung P, et al (1991a) Rapid in vivo imaging of arterial thrombi by Tc-99m labeled anti-activated platelet monoclonal antibody. J Nucl Med, 32: 1005 (abstr)

    Google Scholar 

  • Coller BS (1985) A new murine monoclonal antibody reports an activation-dependent change in the conformation and/or microenvironment of the platelet glycoprotein IIb/IIIa complex. J Clin Invest, 76: 101

    Article  PubMed  CAS  Google Scholar 

  • Coller BS, Peerschke EI, Scudder LE, et al (1983) A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and or IIIa. J Clin Invest, 72: 325

    Article  PubMed  CAS  Google Scholar 

  • Collet B, Moisan A, Maros S, et al (1991) A new In-111 labeled F(ab’) antigranulocyte antibody for imaging focal sites of infection: In vitro studies and preliminary results. J Nucl Med, 32: 1051 (abstr)

    Google Scholar 

  • Desai AG, Thakur ML (1986) Radiolabeled blood cells: Techniques and applications. CRC Critical Rev Clin Lab Sci, 24: 95

    Article  CAS  Google Scholar 

  • Ezekowitz MD, Coller B, Srivastava SC (1986) Potential application of labeled antibodies for thrombus detection. Nucl Med Biol, 13: 407

    CAS  Google Scholar 

  • Hanna R, Braun T, Levendel A, et al (1984) Radiochemistry and biostability of autologous leucocytes labeled with 99mTc-stannous colloid in whole blood. Eur J Nucl Med, 9: 216

    Article  PubMed  CAS  Google Scholar 

  • Hasler PH, Seybold K, Andres RY, et al (1988) Immunoscintigraphic localization of inflammatory lesions: Pharmacokinetics and estimated absorbed radiation dose in man. Eur J Nucl Med, 13: 594

    Article  PubMed  CAS  Google Scholar 

  • Hnatowich DJ, Layne WW, Childs RL, et al (1983) Radioactive labeling of antibody: A simple and efficient method. Science, 220: 613

    Article  PubMed  CAS  Google Scholar 

  • Joseph K, Höfken H, Bosslet K, et al (1988) In vivo labelling of granulocytes with 99mTc anti-NCA monoclonal antibodies for imaging inflammation. Eur J Nucl Med, 14: 367

    Article  PubMed  CAS  Google Scholar 

  • Knight LC, Maurer AH, Ammar IA, et al (1988) Evaluation of indium-111 labeled anti-fibrin antibody for imaging vascular thrombi. J Nucl Med, 29: 494

    PubMed  CAS  Google Scholar 

  • Locher JT, Frey LD, Seybold K, et al (1991) Dual isotope immunoscintigraphy of infections. Eur J Nucl Med, 15:555 (abstr)

    Google Scholar 

  • Locher JT, Seybold K, Andres RY, et al (1986) Imaging of inflammatory lesions after injection of radioiodinated monoclonal antigranulocyte antibodies. Nucl Med Commun, 7: 659

    PubMed  CAS  Google Scholar 

  • McAfee JG, Grossman ZD, Rosebrough SF, et al (1988) Monoclonal antibody against human fibrin for imaging thrombi. In: Radiolabeled Monoclonal Antibodies for Imaging and Therapy, Edited by S.C. Srivastava, Plenum, New York, pp. 807–815

    Chapter  Google Scholar 

  • McAfee JG, Gagne G, Subramanian G (1988a) Radioactive monoclonal antibodies against cell surface antigens for labeling leukocyte subpopulations. In: Radiolabeled Monoclonal Antibodies for Imaging and Therapy, Edited by S.C. Srivastava, Plenum, New York, pp. 795–805

    Chapter  Google Scholar 

  • Oster ZH, Srivastava SC, Som P, et al (1985) Thrombus radioimmunoscintigraphy. An approach using monoclonal antiplatelet antibody. Proc Natl Acad Sci, USA 82: 3465

    Article  PubMed  CAS  Google Scholar 

  • Palabrica TM, Furie BC, Konstam MA, et al (1989) Thrombus imaging in a primate model with antibodies specific for an external membrane protein of activated platelets. Proc Natl Acad Sci, USA 86: 1036

    Article  PubMed  CAS  Google Scholar 

  • Patrick ST, Glowniak JV, Turner FE, et al (1991) Comparison of in vitro RBC labeling with the UltraTagR RBC kit versus in vivo labeling. J Nucl Med, 32: 242

    PubMed  CAS  Google Scholar 

  • Peters AM, Lavender JP, Danpure H, et al (1986) Clinical experience with Tc-99m HMPAO for labeling leukocytes and imaging inflammation. Lancet, 2: 946

    Article  PubMed  CAS  Google Scholar 

  • Peters AM, Lavender JP, Needham SG, et al (1986a) Imaging thrombus with radiolabeled monoclonal antibody to platelets. Brit Med J, 293: 1525

    Article  CAS  Google Scholar 

  • Schwarz A, Steinsträsser A (1987) A novel approach to Tc-99m-labeled monoclonal antibodies. J Nucl Med, 28: 721

    Google Scholar 

  • Seybold K, Locher JT, Coosemans C, et al (1988) Immunoscintigraphic localization of inflammatory lesions: Clinical experience. Eur J Nucl Med, 13: 587

    Article  PubMed  CAS  Google Scholar 

  • Som P, Oster ZH, Zamora PO, et al (1986) Radioimmunoimaging of experimental thrombi in dogs using technetium-99m labeled monoclonal antibody fragments reactive with human plateletes. J Nucl Med, 27: 1315

    PubMed  CAS  Google Scholar 

  • Srivastava SC, Mease RC (1991) Progress in research on ligands, nuclides and techniques for labeling monoclonal antibodies. Nucl Med Biol, 18: 589

    CAS  Google Scholar 

  • Srivastava SC, Meinken GE (1988) Radiolabeled antiplatelet monoclonal antibodies for the scintigraphic localization of in vivo thrombi and vascular lesions. In: Radiolabeled Monoclonal Antibodies for Imaging and Therapy, Edited by Srivastava SC, Plenum, New York 1988, pp. 817–830

    Chapter  Google Scholar 

  • Srivastava SC, Meinken GE, Som P, et al (1984) Evaluation of 123I and 111In labeled monoclonal antiplatelet antibody for the scintigraphic localization of in-vivo thrombi. J Nucl Med, 25:P65 (abstr)

    Google Scholar 

  • Srivastava SC, Straub RF (1990) Blood cell labeling with 99mTc: Progress and perspectives. Semin Nucl Med, 20: 41

    Article  PubMed  CAS  Google Scholar 

  • Srivastava SC, Straub RF, Richards P (1984a) Mechanistic aspects of the technetium-99m-RBC labeling reactions. J Labeled Compds Radiopharm, 21: 1055

    Google Scholar 

  • Steinsträsser A, Berberich R, Schwarz A, et al (1989) Strahlenexposition bei der Szintigraphie mit Tc-99m-Antigranulozyten-Antikörpern. Nuklearmedizin, 28:148

    Google Scholar 

  • Stuttle AWJ, Peters AM, Loutfi I, et al (1988) Use of an anti-platelet monoclonal antibody F(ab’)2 fragment for imaging thrombus. Nucl Med Commun, 9: 647

    Article  PubMed  CAS  Google Scholar 

  • Thakur ML (1990) Immunoscintigraphic imaging of inflammatory lesions: Preliminary findings and future possiblities. Semin Nucl Med, 20: 92

    Article  PubMed  CAS  Google Scholar 

  • Thakur ML (1991) Cell labeling in radiopharmacy: What can radiolabeled monoclonal antibodies offer? Proceedings 4th European Symposium on Radiopharmacy and Radiopharmaceuticals, Baden, Switzerland, May 1-4, 1991, Westera G. and Schubiger, PA, editors, Kluwer Academic Publishers, Dordrecht, NL (In press)

    Google Scholar 

  • Thakur ML, DeFulvio J, Richard MD, et al (1990a) Technetium-99m labeled monoclonal antibodies: Evaluation of reducing agents. Nucl Med Biol, 18: 227

    Google Scholar 

  • Thakur ML, Richard MD, White FW III (1988) Monoclonal antibodies as agents for selective radiolabeling of human neutrophils. J Nucl Med, 29: 1817

    PubMed  CAS  Google Scholar 

  • Thakur ML, Thiagarajan P, White F, et al (1987) Monoclonal antibodies for specific cell labeling: Considerations, preparations, and preliminary evaluation. Nucl Med Biol, 14:51

    CAS  Google Scholar 

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Srivastava, S.C. (1994). The Antibody Approach of Labeling Blood Cells. In: Martin-Comin, J., Thakur, M.L., Piera, C., Roca, M., Lomeña, F. (eds) Radiolabeled Blood Elements. NATO ASI Series, vol 262. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2462-5_4

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  • DOI: https://doi.org/10.1007/978-1-4615-2462-5_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6048-3

  • Online ISBN: 978-1-4615-2462-5

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