Abstract
Although the science of blood cell labeling using monoclonal antibodies directed against specific cellular antigens is still in its early stages, considerable progress has recently been accomplished in this area. The monoclonal antibody approach offers the promise of greater selectivity and enhanced convenience since specific cell types can be labeled in vivo, thus eliminating the need for complex and damaging cell separation procedures. This article focuses on these developments with primary emphasis on antibody labeling of platelets and leukocytes. The advantages and the shortcomings of the recently reported techniques are critically assessed and evaluated.
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References
Andres RY, Seybold K, Tiefenauer L, et al (1988) Radioimmunoscintigraphic localization of inflammatory lesions: Concept, radiolabeling and in vitro testing of a granulocyte antibody. Eur J Nucl Med, 13:582
Baum RP, Hentel A, Bosslet K, et al (1990) First use of Tc-99m labeled monoclonal antibody directed against the T cell receptor for immunoscintigraphic detection of transplant rejection. Eur J Nucl Med, 16:163 (abstr)
Becker W (1990) Immunoscintigraphy of infectious lesions. In: Immunoscintigraphy: Facts and Fictions, edited by D.L. Munz and D. Emrich, Elsevier, Amsterdam, pp. 159–171
Becker W, Borst U, Fischbach W, et al (1989) Kinetic data of in-vivo-labelled granulocytes in humans with a murine Tc-99m-labelled monoclonal antibody. Eur J Nucl Med, 15: 361
Becker W, Horneff G, Emmrich F, et al (1990a) Tc-99m labeled anti CD-4 helper (lymphocytes) antibody scans for imaging of rheumatoid arthritis. Eur J Nucl Med, 16:401 (abstr)
Becker W, Saptogino A, Wolf F (1991) Diagnostic accuracy of a late single Tc-99m-granulocyte antibody scan in inflammatory or infectious diseases. J. Nucl Med, 32: 1002 (abst)
Berberich R, Sutter M, Oberhausen E (1991) Inflammation detection with Tc-99m labeled monoclonal antibody for granulocytes. Dialogos (Siemens), 3/91: pp. 6–11
Bosslet K, Lüben G, Schwarz A, et al (1985) Immunohistochemical localization and molecular characteristics of three monoclonal antibody-defined epitopes detectable on carcinoembryonic antigen (CEA). Int J Cancer, 36:75
Buchegger F, Schreyer M, Carrel S, et al (1984) Monoclonal antibodies identify a CEA crossreacting antigen of 95 kD (NCA-95) distinct in antigenicity and tissue distribution from the previously described NCA of 55 kD. Int J Cancer, 33: 643
Chouraqui P, Davidson M, Thomas C, et al (1991) Effect of thrombus age on uptake of Tc-99m labeled anti-activated platelet monoclonal antibody. J Nucl Med, 32:1013 (abstr)
Chouraqui P, Maddahi J, Fung P, et al (1991a) Rapid in vivo imaging of arterial thrombi by Tc-99m labeled anti-activated platelet monoclonal antibody. J Nucl Med, 32: 1005 (abstr)
Coller BS (1985) A new murine monoclonal antibody reports an activation-dependent change in the conformation and/or microenvironment of the platelet glycoprotein IIb/IIIa complex. J Clin Invest, 76: 101
Coller BS, Peerschke EI, Scudder LE, et al (1983) A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and or IIIa. J Clin Invest, 72: 325
Collet B, Moisan A, Maros S, et al (1991) A new In-111 labeled F(ab’) antigranulocyte antibody for imaging focal sites of infection: In vitro studies and preliminary results. J Nucl Med, 32: 1051 (abstr)
Desai AG, Thakur ML (1986) Radiolabeled blood cells: Techniques and applications. CRC Critical Rev Clin Lab Sci, 24: 95
Ezekowitz MD, Coller B, Srivastava SC (1986) Potential application of labeled antibodies for thrombus detection. Nucl Med Biol, 13: 407
Hanna R, Braun T, Levendel A, et al (1984) Radiochemistry and biostability of autologous leucocytes labeled with 99mTc-stannous colloid in whole blood. Eur J Nucl Med, 9: 216
Hasler PH, Seybold K, Andres RY, et al (1988) Immunoscintigraphic localization of inflammatory lesions: Pharmacokinetics and estimated absorbed radiation dose in man. Eur J Nucl Med, 13: 594
Hnatowich DJ, Layne WW, Childs RL, et al (1983) Radioactive labeling of antibody: A simple and efficient method. Science, 220: 613
Joseph K, Höfken H, Bosslet K, et al (1988) In vivo labelling of granulocytes with 99mTc anti-NCA monoclonal antibodies for imaging inflammation. Eur J Nucl Med, 14: 367
Knight LC, Maurer AH, Ammar IA, et al (1988) Evaluation of indium-111 labeled anti-fibrin antibody for imaging vascular thrombi. J Nucl Med, 29: 494
Locher JT, Frey LD, Seybold K, et al (1991) Dual isotope immunoscintigraphy of infections. Eur J Nucl Med, 15:555 (abstr)
Locher JT, Seybold K, Andres RY, et al (1986) Imaging of inflammatory lesions after injection of radioiodinated monoclonal antigranulocyte antibodies. Nucl Med Commun, 7: 659
McAfee JG, Grossman ZD, Rosebrough SF, et al (1988) Monoclonal antibody against human fibrin for imaging thrombi. In: Radiolabeled Monoclonal Antibodies for Imaging and Therapy, Edited by S.C. Srivastava, Plenum, New York, pp. 807–815
McAfee JG, Gagne G, Subramanian G (1988a) Radioactive monoclonal antibodies against cell surface antigens for labeling leukocyte subpopulations. In: Radiolabeled Monoclonal Antibodies for Imaging and Therapy, Edited by S.C. Srivastava, Plenum, New York, pp. 795–805
Oster ZH, Srivastava SC, Som P, et al (1985) Thrombus radioimmunoscintigraphy. An approach using monoclonal antiplatelet antibody. Proc Natl Acad Sci, USA 82: 3465
Palabrica TM, Furie BC, Konstam MA, et al (1989) Thrombus imaging in a primate model with antibodies specific for an external membrane protein of activated platelets. Proc Natl Acad Sci, USA 86: 1036
Patrick ST, Glowniak JV, Turner FE, et al (1991) Comparison of in vitro RBC labeling with the UltraTagR RBC kit versus in vivo labeling. J Nucl Med, 32: 242
Peters AM, Lavender JP, Danpure H, et al (1986) Clinical experience with Tc-99m HMPAO for labeling leukocytes and imaging inflammation. Lancet, 2: 946
Peters AM, Lavender JP, Needham SG, et al (1986a) Imaging thrombus with radiolabeled monoclonal antibody to platelets. Brit Med J, 293: 1525
Schwarz A, Steinsträsser A (1987) A novel approach to Tc-99m-labeled monoclonal antibodies. J Nucl Med, 28: 721
Seybold K, Locher JT, Coosemans C, et al (1988) Immunoscintigraphic localization of inflammatory lesions: Clinical experience. Eur J Nucl Med, 13: 587
Som P, Oster ZH, Zamora PO, et al (1986) Radioimmunoimaging of experimental thrombi in dogs using technetium-99m labeled monoclonal antibody fragments reactive with human plateletes. J Nucl Med, 27: 1315
Srivastava SC, Mease RC (1991) Progress in research on ligands, nuclides and techniques for labeling monoclonal antibodies. Nucl Med Biol, 18: 589
Srivastava SC, Meinken GE (1988) Radiolabeled antiplatelet monoclonal antibodies for the scintigraphic localization of in vivo thrombi and vascular lesions. In: Radiolabeled Monoclonal Antibodies for Imaging and Therapy, Edited by Srivastava SC, Plenum, New York 1988, pp. 817–830
Srivastava SC, Meinken GE, Som P, et al (1984) Evaluation of 123I and 111In labeled monoclonal antiplatelet antibody for the scintigraphic localization of in-vivo thrombi. J Nucl Med, 25:P65 (abstr)
Srivastava SC, Straub RF (1990) Blood cell labeling with 99mTc: Progress and perspectives. Semin Nucl Med, 20: 41
Srivastava SC, Straub RF, Richards P (1984a) Mechanistic aspects of the technetium-99m-RBC labeling reactions. J Labeled Compds Radiopharm, 21: 1055
Steinsträsser A, Berberich R, Schwarz A, et al (1989) Strahlenexposition bei der Szintigraphie mit Tc-99m-Antigranulozyten-Antikörpern. Nuklearmedizin, 28:148
Stuttle AWJ, Peters AM, Loutfi I, et al (1988) Use of an anti-platelet monoclonal antibody F(ab’)2 fragment for imaging thrombus. Nucl Med Commun, 9: 647
Thakur ML (1990) Immunoscintigraphic imaging of inflammatory lesions: Preliminary findings and future possiblities. Semin Nucl Med, 20: 92
Thakur ML (1991) Cell labeling in radiopharmacy: What can radiolabeled monoclonal antibodies offer? Proceedings 4th European Symposium on Radiopharmacy and Radiopharmaceuticals, Baden, Switzerland, May 1-4, 1991, Westera G. and Schubiger, PA, editors, Kluwer Academic Publishers, Dordrecht, NL (In press)
Thakur ML, DeFulvio J, Richard MD, et al (1990a) Technetium-99m labeled monoclonal antibodies: Evaluation of reducing agents. Nucl Med Biol, 18: 227
Thakur ML, Richard MD, White FW III (1988) Monoclonal antibodies as agents for selective radiolabeling of human neutrophils. J Nucl Med, 29: 1817
Thakur ML, Thiagarajan P, White F, et al (1987) Monoclonal antibodies for specific cell labeling: Considerations, preparations, and preliminary evaluation. Nucl Med Biol, 14:51
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Srivastava, S.C. (1994). The Antibody Approach of Labeling Blood Cells. In: Martin-Comin, J., Thakur, M.L., Piera, C., Roca, M., Lomeña, F. (eds) Radiolabeled Blood Elements. NATO ASI Series, vol 262. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2462-5_4
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DOI: https://doi.org/10.1007/978-1-4615-2462-5_4
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