Abstract
The high sensitivity and specificity of the 111In labeled WBC method make it the “gold standard” against which all the new methods must be measured. However the 111In WBC method requires laborious separation, labeling and reinjection of 111In oxine labeled autologous leukocytes. With the increasing incidence of AIDS there is now the possibility of misadministration of HIV infected cells with disastrous consequences. Radiation exposure from a diagnostic misadministration pales into insignificance by comparison. There is also a small but finite chance of self-infection from a needle stick. These reasons, along with simplicity, are all persuasive arguments for the continued development of kit preparations wherever possible. Thus new approaches amenable to kit formulations, such as human polyclonal immuno-globulin and anti-leukocyte MABsa labeled with either 99mTc or 111In are very attractive alternatives.
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References
Fischman AJ, Rubin RH, Khaw BA, et al. Detection of acute inflammation with 111In-labelled non-specific polyclonal IgG. Semin Nucl Med; 18: 335–344 (1988).
Buscome JR, Lui D, Ensing G, de Jong R, Ell PJ. 99mTc-human immunoglobulin (HIG)-first results of a new agent for the localization of infection and inflammation. Eur J Nucl Med; 16: 649–655 (1990).
McAfee JG, Gagne G, Subramanian G, and Schneider RF. The localization of Indium-I11-leucocytes, Gallium-67-Polyclonal IgG and other radioactive agents in acute focal inflammatory lesions. J Nucl Med; 32: 2126–2131 (1991).
Reardon DT, Meares CF, Goodwin DA, et al: Antibodies against metal chelates. Nature; 316: 265–268 (1985).
Goodwin DA, Meares CF, McCall MJ, McTigue M, Chaovapong W. Pre-targeted immunoscintigraphy of murine tumors with indium-111 labeled bifunctional haptens. J Nucl Med;, 29: 226–234 (1988).
Goodwin DA, Meares CF, McCall MJ, Chaovapong W, McTigue M, Diamante CI: Pretargeted immunoscintigraphy with In-111, Ga-67 and Ga-68 labeled bivalent (“Janus”) haptens. J Clin Nuc Med, 13:9Spl0 (Abstract A3) (1988).
Le Doussal M, Martin M, Gautherot E, Delaage M, and Barbet J. In vitro and in vivo targeting of radiolabeled monovalent and divalent haptens with dual specificity monoclonal antibody conjugates: enhanced divalent hapten affinity for cell-bound antibody conjugate. J Nucl Med; 30: 1358–1366 (1989).
Karush F. Multivalent binding and functional affinity. Contemp Top Mol Immunol; 5: 217 (1976).
Goodwin DA. Strategies for antibody targeting. Antibody, Immunoconjugates, and Radiopharmaceuticals; 4(4): 427–434 (1991).
Hnatowitch DJ, Virzi F, and Ruskovski M. Investigations of avidin and biotin for imaging applications. J Nucl Med;, 28:1294–1302 (1987).
Goodwin DA, Meares CF, McCall MJ, McTigue M. An avidin-biotin chelate system for imaging tumors. J Nucl Med; 28:722 (abst) (1987).
Paganelli G, Magnani P, Zito F, et al. Three-step monoclonal antibody tumor targeting in carcinoembryonic antigen-positive patients. Ca Res; 51: 5960–5966 (1991).
Goodwin DA, Meares CF, McTiue M, Caovapong W, Ransone C, Renn O, McCall MJ, Studer M. Pharmacokinetics of biotin-chelate conjugates for pretargeted avidin-biotin immunoscintigraphy. J Nucl Med; 33: 880 [abstract] (1992).
Rusckowski M, Paganelli G, Hnatowich DJ, Virzi F, Fogarasi M, Fazio F. Imaging infection/inflammation in patients with streptavidin and radiolabeled biotin: preliminary observations. J Nucl Med; 33: 924 [abstract] (1992).
Ruskovsky M, Fritz BS, Hnatowich DJ. Localization of infection using streptavidin and biotin: an alternative to nonspecific polyclonal IgG. J Nucl Med; 33: 1810–1815 (1992).
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Goodwin, D.A. et al. (1994). Advances in Blood Cell Labeling: Pretargeting. In: Martin-Comin, J., Thakur, M.L., Piera, C., Roca, M., Lomeña, F. (eds) Radiolabeled Blood Elements. NATO ASI Series, vol 262. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2462-5_1
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