Abstract
Nowadays, the polymerase chain reaction (PCR) is the most sensitive and widely used method for the detection of human papillomavirus (HPV) using ethidium bromide gel electrophoresis together with hybridization assays for typing. In this study, we demonstrate single-strand conformational. polymorphism (SSCP) as simultaneous detection and typing method for PCR-amplified products. SSCP as first described by Orita et al.l,2 employs denaturation of double strand DNA into single strand DNA and separation of the products by polyacrylamide gel electrophoresis (PAGE). This method makes possible the detection of HPV-DNA conformational. changes resulting in indivual. band patterns of the most common HPV-types.
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References
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© 1994 Springer Science+Business Media New York
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Zehbe, I., Rylander, E., Sällström, J., Wilander, E. (1994). Genotyping of Human Papillomavirus (HPV) by Single-Strand Conformational Polymorphism (SSCP). In: Stanley, M.A. (eds) Immunology of Human Papillomaviruses. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2449-6_10
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DOI: https://doi.org/10.1007/978-1-4615-2449-6_10
Publisher Name: Springer, Boston, MA
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Online ISBN: 978-1-4615-2449-6
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