Abstract
An enzyme sensor for glucose fabricated by the electrochemical polymerization method is described. Electropolymerized films of 2,2’-bithiophene is used to immobilized glucose oxidase at platinum electrodes. The responses of the enzyme-polymer electrodes toward glucose are analyzed under various conditions. The relation between performance of the polymer-modified electrode and properties of the polymer formed is also discussed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
R.Wilson and A.P.F. Turner, Glucose oxidase: an ideal enzyme, Biosens. Bioelectron. 7: 165(1992).
G. Marko-Varga, R. Appelqvist and L.Gorton, A glucose sensor based on glucose dehydrogenase absorbed on a modified carbon electrode, Anal. Chem. Acta 179:371(1986).
Y. Kajiya, H. Matsumoto and H. Yoneyama, Glucose sensitivity of poly(pyrrole) containing immobilized glucose dehydrogenase, nicotinamide adenine dinucleotide, and β-naphthoquinonesulphonate ions, J. Electroanal. Chem. 319: 185 (1991).
P.N. Bartlett and R.G. Whitaker, Strategies for the development of amperometric enzyme electrodes, Biosensors 3: 359(1987/88).
N.C.Fould and C.R.Lowe, Enzyme entrapment in electrically conducting polymers: immobilization of glucose oxidase in in polypyrrole and its application in amperometric glucose sensors, J. Chem. Soc., Faraday Trans. 1 82: 1259(1986).
M. Umana and J. Waller, Protien modified electrodes: glucose oxidase/polypyrrole system, Anal. Chem. 58: 2979(1986).
E. Tamiya, I. Karube, S. Hattori, M. Suzuki and K. Yokoyama, Micro glucose sensors using electron mediators immobilized on a polypyrrole-modified electrode, Sens. Actuators 18: 297(1989).
J. Rishpon and S. Gottesfeld, Investigation of polypyrrole/glucose oxidase electrodes by ellipsometric, microgravimetric and electrochemical measurements, Biosens. Bioelectron. 6: 143(1991).
P.N. Bartlett and R.G. Whitaker, Electrochemical immobilization of enzymes part II. glucose oxidase immobilized in poly-N-metylpyrrole, J. Electroanal. Chem. 224: 37(1987).
H. Shinihana, T. Chiba and M. Aizawa, Enzyme microsensor for glucose with an electrochemically synthesized enzyme-polyaniline film, Sens. Actuators 13: 79(1988).
S.Mu, H.Xue and B.Qian, bioelectrochemical responses of the polyaniline glucose oxidase electrode, J. Electroanal. Chem. 304: 7(1991).
H.S.O. Chan, S.C. Ng, S.H. Seow and M.J.G. Moderscheim, Symmetrically disubstituted poly(bithiophene): influence of the halogen substituents, J. Mater. Chem. 2: 11: 1135 (1992).
A.R.Hillman and E.F.Mallen, Nucleation and growth of polythiophene films on gold electrode, J. Electroanal. Chem. 220: 351 (1987).
P.N.Bartlett and R.G.Whìtaker, Electrochemical immobilization of enzymes part I. theory, J. Electroanal. Chem. 224: 27(1987).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media New York
About this chapter
Cite this chapter
Chan, H.S.O., Ng, SC., Seow, SH., Chi, H. (1994). Immobilization of Glucose Oxidase in Poly(2,2′-bithiophene). In: Prasad, P.N. (eds) Frontiers of Polymers and Advanced Materials. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2447-2_31
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2447-2_31
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6040-7
Online ISBN: 978-1-4615-2447-2
eBook Packages: Springer Book Archive