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Humanization of an Anti-Mucin Antibody for Breast and Ovarian Cancer Therapy

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Antigen and Antibody Molecular Engineering in Breast Cancer Diagnosis and Treatment

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 353))

Summary

Antibody-drug conjugates utilize the targetting potential of antibodies to improve the potential of cytostatic or cytocidal drugs. One such murine monoclonal antibody, CTM01 (mCTM0l), which recognizes an epitope on breast epithelial mucin, has potential for the treatment of breast and ovarian cancers. We examine in this paper the comparative properties of mCTM01 against a number of other anti-mucin antibodies. We then describe the humanization and high level re-expression of humanized CTM01 (hCTM0l), a process designed to avoid the immune response to administered murine antibodies in human patients and to produce sufficient material for clinical studies. We show that the humanized form has properties superior to mCTM01 in terms of binding affinity to antigen presented on tumour cells.

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Authors and Affiliations

  1. Oncology Biology, Celltech Research Division Celltech Ltd., 216 Bath Rd., Slough, Berks, UK, SL1 4EN

    T. S. Baker, H. M. Caskey-Finney, D. J. King, A. D. G. Lawson, A. Lyons, A. Mountain, M. R. Rolfe, M. Sehdev, G. T. Yarranton & J. R. Adair

  2. Oncology Chemistry, Celltech Research Division Celltech Ltd., 216 Bath Rd., Slough, Berks, UK, SL1 4EN

    C. C. Bose

  3. Inflammation Biology, Celltech Research Division Celltech Ltd., 216 Bath Rd., Slough, Berks, UK, SL1 4EN

    R. J. Owens

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  1. Cancer Research Fund of Contra Costa, Walnut Creek, California, USA

    Roberto L. Ceriani

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Baker, T.S. et al. (1994). Humanization of an Anti-Mucin Antibody for Breast and Ovarian Cancer Therapy. In: Ceriani, R.L. (eds) Antigen and Antibody Molecular Engineering in Breast Cancer Diagnosis and Treatment. Advances in Experimental Medicine and Biology, vol 353. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2443-4_8

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  • DOI: https://doi.org/10.1007/978-1-4615-2443-4_8

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