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Genetic Engineering of Oat

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Improvement of Cereal Quality by Genetic Engineering

Summary

Fertile, transgenic oat (Avena sauva L.) plants were regenerated from approximately 35% of phosphinothricin (PPT)-resistant callus cultures selected following microprojectile bombardment to deliver the plasmid pBARGUS. The plasmid pBARGUS contains the bar gene, which confers plant cell resistance to PPT and related herbicides, and the uidA gene for β-glucuronidase (GUS) under the control of the maize alcohol dehydrogenase I promoter. This promoter conferred high levels of GUS activity in the endosperm of mature oat kernels, thus enabling ready determination of transmission genetics of the GUS transgene. Segregation ratios of GUS activity in mature R1 seed and, in some cases, the R2 and R3 generations were determined in 15 transgenic families. Seven families fit a 3: 1 GUS+:GUS- segregation ratio whereas two families segregated 15: 1 for GUS activity. The remaining six families exhibited aberrant segregation ratios. These initial studies used friable, embryogenic callus initiated from immature embryos of a specific genotype selected for high frequency callus initiation. Although this system was useful in establishing and characterizing oat transformation, its limitation to a specific genotype and the undesirability of herbicide tolerance in oat dictated further development of transformation systems for use in oat improvement. Transformation of current oat cultivars and the use of an antibiotic-based selection system to obviate the herbicide resistance marker are described.

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Author information

Authors and Affiliations

  1. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, 55108, USA

    D. A. Somers, K. A. Torbert, W. P. Pawlowski & H. W. Rines

  2. Plant Science Research Unit U.S. Department of Agriculture, Agriculture Research Service, St. Paul, Minnesota, 55108, USA

    D. A. Somers, K. A. Torbert, W. P. Pawlowski & H. W. Rines

Authors
  1. D. A. Somers
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  2. K. A. Torbert
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  3. W. P. Pawlowski
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  4. H. W. Rines
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Editor information

Editors and Affiliations

  1. Queensland Agricultural Biotechnology Centre Gehrmann Laboratories, University of Queensland, St. Lucia, Queensland, 4072, Australia

    Robert J. Henry

  2. Division of Plant Industry, CSIRO, North Ryde, NSW, Australia

    John A. Ronalds

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© 1994 Springer Science+Business Media New York

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Somers, D.A., Torbert, K.A., Pawlowski, W.P., Rines, H.W. (1994). Genetic Engineering of Oat. In: Henry, R.J., Ronalds, J.A. (eds) Improvement of Cereal Quality by Genetic Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2441-0_6

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  • DOI: https://doi.org/10.1007/978-1-4615-2441-0_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6037-7

  • Online ISBN: 978-1-4615-2441-0

  • eBook Packages: Springer Book Archive

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