Abstract
Barley quality encompasses a range of chemical and physical attributes which depend on whether the grain is to be used in the preparation of malt for the brewery, as a component of stockfeed formulations, or in human nutrition. Currently, specifications for barley quality are tailored primarily for the malting and brewing industries, although agronomic properties such as yield and disease resistance are also important selection criteria in breeding programs. Thus, parameters such as grain size, dormancy, malt extract, grain protein content, development of hydrolytic enzymes for starch degradation (diastatic power), apparent attenuation levels, (1 → 3, 1 → 4)-β-glucanase potential and (1 → 3, 1 → 4)-β-glucan content represent commonly used quality indicators. Many of these quality characteristics are determined by the expression of several or many individual genes, such that the genetic manipulation of the characters by recombinant DNA technology requires a thorough understanding of the individual genes, their interactions in the expression of a particular quality characteristic, or the identification of the rate-limiting component of the characteristic. Quality factors that are determined by a single gene are more amenable to manipulation by genetic engineering and there are developing technologies through which the expression of individual genes can be either enhanced or inhibited, depending on the desired effect on malting quality. In this paper, the potential for engineering barley to enhance (1 → 3, 1 → 4)-β-glucanase activity is explored, together with the biochemical, genetic and physiological information that necessarily underpins genetic engineering technology.
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References
Chen, L., Garrett, T.J.P., Varghese, J.N., Fincher, G.B. and Høj, P.B. (1993) Crystallization and preliminary X-ray analysis of (1,3)-and (1 → 3;1 → 4)-β-D-glucanases from germinating barley. J. Mol. Biol, in the press.
Doan, D.N.P. and Fincher, G.B. (1992) Differences in the thermostabilities of barley (1 → 3, 1 →4)-β-glucanases are only partly determined by N-glycosylation. FEBS Letters, 309, 265–271.
Lemaux, P., Wan, Y and Williams, R. (1993) Development and Use of Transformation Systems for Maize & Barley for Agronomic Improvement. Chem. in Ausl., 60, 496.
Litts, J.C., Simmons, C.R., Karrer, E.E., Huang, N. and Rodriguez, R.L. (1990) The isolation and characterization of a barley (1 → 3, 1 → 4)-β-glucanase gene. Eur. J. Biochem. 194, 831–838.
Loi, L., Barton, P.A. and Fincher, G.B. (1987) Survival of barley (1 → 3, 1 → 4)-β-glucanase isoenzymes during kilning and mashing. J. Cereal Sci. 5, 45–50.
Slakeski, N., Baulcombe, D.C., Devos, K.M., Doan, D.N.P. and Fincher, G.B. (1990) Structure and tissue-specific regulation of genes encoding barley (1 → 3, 1 → 4)-β-glucan endohydrolases. Mol. Gen. Genet. 224, 437–449.
Stuart, I.M., Loi, L. and Fincher, G.B. (1988) Varietal and environmental variations in (1 → 3, 1 →4)-β-glucan levels and (1 → 3, 1 → 4)-β-glucanase potential in barley: relationships to malting quality. J. Cereal Sci. 7, 61–71.
Wolf, N. (1991) Complete nucleotide sequence of a Hordeum vulgare gene encoding (( 1 → 3, 1 →4)-β-glucanase isoenzyme IL Plant Physiol. 96, 1382–1384.
Woodward, J.R. and Fincher, G.B. (1982) Purification and chemical properties of two (1, 3: l,4)-β-endohydrolases from germinating barley. Eur. J. Biochem. 121, 663–669.
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© 1994 Springer Science+Business Media New York
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Fincher, G.B. (1994). Potential for the Improvement of Malting Quality of Barley by Genetic Engineering. In: Henry, R.J., Ronalds, J.A. (eds) Improvement of Cereal Quality by Genetic Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2441-0_16
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DOI: https://doi.org/10.1007/978-1-4615-2441-0_16
Publisher Name: Springer, Boston, MA
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