Summary
Pre-harvest sprouting results in significant loss of end-use quality in wheat. Several biochemical events are associated with the deterioration in quality. The production of enzymes associated with mobilisation of endosperm reserves during germination is a major factor. Several approaches to control of sprouting damage by genetic engineering have been considered. The expression of proteins capable of inhibiting the action of hydrolytic enzymes is an important option. Progress has been made towards the production of trans-genic wheat with genes encoding inhibitors of the main starch degrading enzyme, alpha-amylase.
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References
Battershell, V.G. and Henry, R.J. (1990) High performance liquid chromatography of alpha-amylases from germinating wheat and complexes with the alpha-amylase inhibitor from barley. J. Cereal Sci. 12, 73–81.
Derera, N. (1990) A perspective of sprouting research. Fifth International Symposium on Pre-harvest Sprouting in Cereals (Ringland, K., Mosleth, E. and Mares, D.J.,Eds.) Westview Press, Boulder 3–11.
Gale, M.D. (1983) Alpha-amylase genes in wheat. Third International Symposium on Pre-harvest Sprouting in Cereals (Kruger, J.E. and LaBerge, D.E., Eds.) Westview Press, Boulder 105–110.
Gale, M.D., Flintham, J.E. and Mares, D.J. (1983) Applications of molecular markers in breeding for low alpha-amylase wheats. Fifth International Symposium on Pre-harvest Sprouting in Cereals (Ringlund, K., Mosleth, E. and Mares, D.J., Eds.) Westview Press, Boulder 167–175.
Haak, I.C., Brennan, P.S., McKinnon, G.E. and Henry, R.J. (1993) Tissue culture as a mechanism for gene transfer from barley to wheat. Tenth Australian Plant Breeding Conference 2, 191–192.
Henry, R.J., Battershell, V.G., Brennan, P.S. and Oono, K. (1992) Control of wheat alpha-amylase using inhibitors from cereals. J. Sci. Food Agric. 58, 281–284.
Henry, R.J. and Blakeney, A.B. (1990) Post harvest management of alpha-amylase. Aspects of Applied Biology 25, 387–393.
Henry, R. J., McKinnon, G.E., Haak, I.C. and Brennan, P.S. (1993) Use of alpha-amylase inhibitors to control sprouting. Preharvest Sprouting in Cereals 1992, (Walker-Simmons, M.K. and Ried, J.L.,Eds) American Association of Cereal Chemists, St Paul 232–235.
Henry, R.J., Martin, D.J. and Blakeney, A.B. (1987) Reduction in the alpha-amylase content of sprouted wheat by pearling and milling. J. Cereal Sci. 5, 155–166.
Henry, R.J. and Oono, K. (1991) Amplification of a GC-rich sequence from barley by a two-step polymerase chain reaction in glycerol. Plant Mol. Biol. Rep. 9, 139–144.
Jacobsen, J.V. and Close, T.J. (1991) Control of transient expression of chimaeric genes by gibberellic acid and abscisic acid in protoplasts prepared from mature barley aleurone layers. Plant Mol. Biol. 16, 713–724.
Kruger, J.E. (1990) Monitoring for sprouting damage in cereals and its implications for end-product quality. Fifth International Symposium on Pre-harvest Sprouting in Cereals. (Ringland, K., Mosleth, E. and Mares, D.J., Eds.) Westview Press, Boulder 321–328.
Leah, R and Mundy, J. (1989) The bifunctional alpha-amylase inhibitor of barley: nucleotide sequence and patterns of seed specific expression. Plant Mol. Biol. 12, 1028–1031.
McMaster, G.J. (1987) Pre-harvest sprouting in wheat—the Australian experience. Fourth International Symposium on Pre-harvest Sprouting in Cereals (Mares, D.J., Ed.) Westview Press, Boulder 3–14.
Moor, T. (1987) Problems encountered in storage of sprouted barley. Fourth International Symposium on Pre-harvest Sprouting in Cereals (Mares, D.J. Ed.) Westview Press, Boulder 212–221.
Mundy, J., Svendsen, I. and Hejgaard, J. (1983) Barley alpha-amylase/subtilisin inhibitor. 1. Isolation and characterisation. Carlsberg Res. Comm. 48, 81–90.
Ringlund, K. (1987) Pre-harvest sprouting in barley. Fourth International Symposium on Pre-harvest Sprouting in Cereals (Mares, D.J. Ed.) Westview Press, Boulder 15–23.
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Henry, R.J., McKinnon, G., Haak, I.A., Brennan, P.S. (1994). Genetic Engineering of Resistance to Starch Hydrolysis Caused by Pre-Harvest Sprouting. In: Henry, R.J., Ronalds, J.A. (eds) Improvement of Cereal Quality by Genetic Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2441-0_15
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DOI: https://doi.org/10.1007/978-1-4615-2441-0_15
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