Abstract
The derived amino acid sequences of human T-cell receptor β chain shows significant homology to λ light chains of immunoglobulins in its variable, joining, and constant regions. We assessed the cross-reactivity between Tcr β chains and immunoglobulin light chains by determining the capacity of rabbit antisera to human or murine immunoglobulins to react to a synthesized set of nested, overlapping 16-mer peptides corresponding to the VDJC sequence of the Tcr β chain YT35. The observed reactivities were consistent with homologies to λ and k light chains, the strongest reactivity being with a peptide that corresponds to the “switch peptide” of light chains, as assessed by ELISA binding and competitive inhibitions assays. Other regions reactive with anti-light chain sera corresponded to CDR1 and Fr3 segments of the variable domain and a segment of the constant region predicted to loop out of the tight globular structure. The peptide immunochemical results, together with the identification of specific regions of sequence correspondence between Tcr β and the characterized λ light chain Mcg, allowed us to develop a 3-dimensional model of the β chain consistent with its role in antigen recognition.
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References
J.J. Marchalonis, and S.F. Schluter, Evolution of variable and constant domains and joining segments of rearranging immunoglobulins. The FASEB Journal,3:2469 (1989).
Y. Yanagi, Y. Yoshikai, K. Leggett, S.P. Clark, I. Aleksander, T.W. Mak, A human T-cell specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains, Nature (Lond), 308:145 (1984).
N.M. Hogg, and M.F. Greaves, Antigen-binding Thymus-derived lymphocytes. II. Nature of the Immunoglobulin Determinants, Immunology 22:967 (1972).
N.L. Warner, Membrane immunoglobulins and antigen receptor on B and T lymphocytes, Adv.Immunol. 19, 67 (1974).
R.E. Cone, M. Feldman, J.J. Marchalonis and G.J.V. Nossal, Cytophilic properties of surface immunoglobulin of thymus-derived lymphocytes. Immunology 26:49 (1974).
J.J. Marchalonis, Lymphocyte surface immunoglobulins: molecular properties and function as receptors for antigen. Science 190:20 (1975).
H. Binz, and H. Wigzell, Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants, J. Exp. Med. 142:197 (1975).
A.B. Edmundson, K.R. Ely, E.E. Abola, M. Schiffer, and N. Pangiotopoulos, Rotational allomerism and divergent evolution of domains in immunoglobulin light chains, Biochemistry 14:3953 (1975).
F. Dedeoglu, R.A. Hubbard, S.F. Schluter, and J.J. Marchalonis, T-cell receptors of man and mouse studied with antibodies against synthetic peptides, Exptl. & Clin. Immunogenetics, 9:95 (1992).
J.J. Marchalonis, F. Dedeoglu, H. Kaymaz, S.F. Schluter, and A.B. Edmundson, Antigenic mapping of a human λ light chain: Correlation with 3-dimensional structure, J. Protein Chemistry, 11:129 (1992).
C.R. Ross, R.A. Hubbard, S.F. Schluter, A. Diamanduros, A.C. Wang, and J.J. Marchalonis, Antibodies to synthetic peptides corresponding to variable region first framework (Fr1) segments of T cell receptors: Detection of T cell products and cross-reactions with classical immunoglobulins, Immunologic Research, 8:81 (1989).
J.J. Marchalonis, H. Kaymaz, F. Dedeoglu, S.F. Schluter, D.E. Yocum, and A.B. Edmundson, Human autoantibodies reactive with synthetic autoantigens from T-cell receptor ß chain, Proc. Natl. Acad. Sci. USA,89:3325 (1992).
A.M. Pullen, J. Bill, R.T. Kubo, P. Marrack, and J.W. Kappler, Analysis of the interaction site for the self-superantigen Mls-1a on T-cell receptor Vß, J. Exp. Med. 173:1183 (1991).
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Kaymaz, H., Dedeoglu, F., Schluter, S.F., Edmundson, A.B., Marchalonis, J.J. (1994). Cross-Reactions of Anti-Immunoglobulin Sera with Synthetic T-Cell Receptor β Peptides: Mapping on a 3-Dimension Model. In: Atassi, M.Z. (eds) Immunobiology of Proteins and Peptides VII. Advances in Experimental Medicine and Biology, vol 347. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2427-4_10
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DOI: https://doi.org/10.1007/978-1-4615-2427-4_10
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