Abstract
Normal cells in culture can be grown for only a limited number of cell divisions after which they exhibit morphological changes and cease proliferation, a process termed cellular senescence or cellular aging (1). Hayflick and Moorhead (2) reported this finding with human fibroblasts over 30 years ago, and it has been subsequently confirmed by many investigators using cells from different tissues and species. The failure of cells to grow beyond this limit is an inherent property of the cells that cannot be explained simply by inadequate media components or growth conditions (1, 2). The key determinant in the life span of cells in culture is the number of cell doublings, not the length of time in culture (1). Normal cells transplanted serially in vivo also exhibit a finite life span, suggesting that cellular senescence is not a cell culture artifact (3). Several lines of evidence suggest that the aging of cells in culture may be related to the aging of the organism (1, 4). These lines of evidence, although not conclusive, provide provocative support for the hypothesis that aging of cells is related to the aging process of the organism.
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Barrett, J.C., Afshari, C.A. (1994). Cellular Senescence and the Cell Cycle. In: Hu, V.W. (eds) The Cell Cycle. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2421-2_9
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DOI: https://doi.org/10.1007/978-1-4615-2421-2_9
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