Expression of G1 Cyclins During Early Development of Zebrafish Embryos
The generation of multicellular organisms requires precise spatio-temporal regulation of cell divisions. The proliferation of cells within the embryo is well coordinated with morphogenesis and differentiation, and the respective regulatory mechanisms vary in different developmental stages. Progress through all phases of the cell cycle, in invertebrates as well as vertebrates, is extensively modified during early embryonic development. For example, in many species such as Xenopus laevis and Drosophila melanogaster, the early cleavages immediately following fertilization are very rapid and consist of alternating S and M phases of the cell cycle, with no detectable G1 or G21, 2, 3. At the midblastula transition, the cell cycle slows dramatically 2, 4. in Drosophila, the three cell cycles following the midblastula transition are composed of S, G2 and M phases in which the length of G2 is regulated by the product of the string gene (cdc25) 5. During subsequent development in Drosophila, in addition to S, G2 and M, cells undergo G1, a phase in which much of the succeeding growth regulation appears to occur 6, 7.
KeywordsMigration Polypeptide DAPI Rhodamine Ponceau
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