Abstract
As cells enter S phase of the growth cycle there is a transient increase in the transcription and translation of a number of genes that prepare the cell for DNA synthesis. Increased levels of enzymes such as dihydrofolate reductase (DHFR), thymidine kinase, thymidylate synthase, ribonucleotide reductase, CAD, and DNA polymerise alpha help to create an environment favorable for DNA synthesis. As is true for most genes critical for cell growth, expression of these genes is controlled on many levels. Our interests are to understand the control mechanisms that lead to increased transcription at the G1/S-phase boundary. Using the DHFR promoter as a model system, we mapped a cis-acting element necessary and sufficient for a transcriptional increase late in G11, 2. We propose a simple model in which the transcription factor E2F1 binds to and activates transcription from this element in a growth-regulated manner. However, this model is not complete and additional factors may play a role in the growth regulation of the DHFR gene.
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Slansky, J.E., Farnham, P.J. (1994). The Role of the Transcription Factor E2F in the Growth Regulation of DHFR. In: Hu, V.W. (eds) The Cell Cycle. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2421-2_16
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DOI: https://doi.org/10.1007/978-1-4615-2421-2_16
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