The Cell Cycle pp 123-125 | Cite as

Histone Gene Transcription During the Cell Cycle

  • Franca LaBella
  • Rosanna Martinelli
  • Neil Segil
  • Nathaniel Heintz
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

It has become evident during the last decade that periodic transcription of a wide variety of mRNAs is a critical component of the eucaryotic cell cycle. The first genes to be identified whose transcription is modulated during cell cycle progression were the histone genes (1). in vertebrates, the histone genes comprise a small gene family in which there are approximately a dozen genes encoding each of the four core histones (H4, H3, H2a, H2b), and approximately half that number encoding the linker histone H1 (2). Since histones are essential structural proteins, they are transcribed in most eucaryotic cells. However, in actively dividing cells their transcription is very tightly coupled to S phase of the cell cycle. Thus, as cells enter S phase, histone gene transcription is increased approximately ten fold, and as the cell exits S phase the rate of histone gene transcription returns to its pre-S phase levels. This tight coupling of histone gene transcription with DNA replication provides an opportunity to dissect molecular mechanisms of transcription that are responsive to specific cues during traverse through the cell cycle, and that may be essential for orderly progression toward division. The interest of our laboratory has been to define these transcriptional regulatory mechanisms, and to relate them to other critical events that are important for cell cycle regulation.

Keywords

Filtration Polypeptide Glutamine Polyacrylamide 

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References

  1. 1.
    Osley, M.A. (1991). Ann. Rev. Biochem. 60: 827–861.PubMedCrossRefGoogle Scholar
  2. 2.
    Wells, J. R. E. (1986). Compilation analysis of histones and histone genes. Nucleic Acids Res. 14: 119–149.CrossRefGoogle Scholar
  3. 3.
    LaBella, F., Sive, H.L., Roeder, R.G. and N. Heintz (1988). Cell-cycle regulation of a human histone gene H2B gene is mediated by the H2B subtype-specific DNA consensus element. Genes and Devel. 2, 32.CrossRefGoogle Scholar
  4. 4.
    Dalton, S. and J. R. E. Wells (1985). A gene specific promoter element is required for optimal expression of the histone H1 gene in S-phase. EMBO J. 7: 49–56.Google Scholar
  5. 5.
    LaBella, F., Gallinai, P., McKinney, J., and N. Heintz (1990). Histone H1 subtype-specific consensus elements mediate cell cycle-regulated transcription in vitro. Genes and Development 3, 1982.CrossRefGoogle Scholar
  6. 6.
    Roberts, S. B., Segil, N. and N. Heintz (1991). Octl (OTF1) is differentially phosphorylated during the cell cycle. Science 253: 1022–1026.PubMedCrossRefGoogle Scholar
  7. 7.
    Segil, N., Roberts, S. B. and N. Heintz (1991). Mitotic phosphorylation of the Octl POU-homeodomainiid regulation of DNA binding. Science 254, 1814.PubMedCrossRefGoogle Scholar
  8. 8.
    Carbon, P., Murgo, S., Ebel, J.-P., Krol, A., Tewbb, G. and I. W. Mattaj (1987). A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerise III and U2 snRNA by RNA polymerise II. Cell 51: 71–79.PubMedCrossRefGoogle Scholar
  9. 9.
    Martinelli, R. and N. Heintz (1993). HlTF2A: the large subunit of a heteromeric, glutamine rich CCAAT-binding transcription factor involved in histone H1 cell cycle regulation. in preparation.Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Franca LaBella
    • 1
  • Rosanna Martinelli
    • 1
  • Neil Segil
    • 1
  • Nathaniel Heintz
    • 1
  1. 1.Howard Hughes Medical Institute Laboratory of Molecular BiologyThe Rockefeller UniversityNew YorkUSA

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