Abstract
Ran/TC4, a Ras-related nuclear protein first identified in a human teratocarcinoma eDNA library, is postulated to function in conjunction with another protein, RCC1 (Regulator of Chromosomal Condensation-1), to prevent the initiation of mitosis before the completion of DNA synthesis. RCC1 is a chromatin-associated DNA-binding protein as well as a Ran/TC4-specific guanine nucleotide exchange factor. One model for the function of Ran/TC4 and RCC1 is that RCC1 monitors DNA synthesis and maintains Ran/TC4 in a GTP-bound form (Ran/TC4 •GTP) until the completion of DNA synthesis and that Ran/TC4 • GTP in turn inhibits the activation of MPF (Mitosis Promoting Factor). We are testing this model, in which a GTPase cycle is coupled to the cell cycle via MPF by ex-pressing mutant Ran/TC4 proteins in human cells, and by using a Ran/TC4 - [32P]GTP complex as a probe to isolate Ran-interacting proteins.
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Coutavas, E., Ren, M., Oppenheim, J.D., Yajnik, V., D’Eustachio, P., Rush, M.G. (1994). A GTPase Cycle Coupled to the Cell Cycle. In: Hu, V.W. (eds) The Cell Cycle. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2421-2_11
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DOI: https://doi.org/10.1007/978-1-4615-2421-2_11
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