Characterization of Rat Lacrimal Gland Protein Kinase C: Effects of Phorbol Esters and PKC Inhibitors on Histone Kinase Activity and Labelled Protein Discharge

  • Driss Zoukhri
  • Philippe Mauduit
  • Bernard Rossignol
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 350)

Abstract

In the rat lacrimal gland, cholinergic agonists induce a large protein secretion through muscarinic receptors. The activation of these receptors stimulates phospholipase C that hydrolyses phosphatidylinositol 4,5-bisphosphate producing inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). The roles of IP3 and DAG as second messengers are now well documented. IP3 releases calcium from a non mitochondrial intracellular store which increases the cytosolic free calcium concentration. The released calcium may stimulate secretion directly, or may act with calmodulin to activate specific kinases that phosphorylate specific proteins to induce secretion. DAG activates protein kinase C (PKC), a calcium and phospholipid-dependent protein kinase, which also may phosphorylates specific proteins to evoke secretion.

Keywords

Magnesium Hydrochloride Leucine Acetyl Hydroxyapatite 

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References

  1. Castagna, M., Takai, Y., Kaibuchi, K., Sano, K., Kikkawa, U. and Nishizuka, Y., 1982, Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumour-promoting phorbol esters, J. Biol. Chem. 257:7847.PubMedGoogle Scholar
  2. Dartt, D.A., Guerina, V.J., Donowitz, M., Taylor, L. and Sharp, G.W.G., 1982, Calcium and calmodulin-dependent protein phosphorylation in rat lacrimal gland, Biochem. J., 202:799.PubMedGoogle Scholar
  3. Dartt, D.A., Baker, L.V., Rose, P.E., Murphy, S.E., Ronco, L.V. and Unser, M.F., 1988a, Role of cyclic AMP and calcium in potentiation of rat lacrimal gland protein secretion. Invest. Ophtalmo. 29:1732.Google Scholar
  4. Dartt, D.A., Ronco, L.V., Murphy, S.A. and Unser, M.F., 1988b, Effect of phorbol esters on rat lacrimal gland protein secretion, Invest. Ophtalmo. 29:1726.Google Scholar
  5. Dartt, D.A., Hodges, R.R. and Dicker, D.M., 1990, Translocation of protein kinase C-ɛ in the rat lacrimal gland, J. Cell. Biol. 11:85a.Google Scholar
  6. Herman, G., Busson, S., Ovtracht, L., Maurs, C. and Rossignol, B., 1978, Regulation of protein discharge in two exocrine glands: rat parotid and exorbital lacrimal glands, Biol. Cell. 31:255.Google Scholar
  7. Jaken, S., 1990, Protein kinase C and tumour promoters, Cur. Op. Cell. Biol. 2:192.CrossRefGoogle Scholar
  8. Mauduit, P., Herman, G. and Rossignol, B., 1983, Effect of trifluoperazine on 3H-labeled protein secretion induced by pentoxyfilline, cholinergic or adrenergic agonists in rat lacrimal gland a possible role of calmodulin?, FEES Lett. 152:207.CrossRefGoogle Scholar
  9. Mauduit, P., Herman, G. and Rossignol, B., 1984, Protein secretion induced by isoproterenol or pentoxyfilline in lacrimal gland: calcium effects, Am. J. Physiol. 264:C37.Google Scholar
  10. Mauduit, P., Herman, G. and Rossignol, B., 1987, Newly synthesized protein secretion in rat lacrimal gland: post-second messenger synergism, Am. J. Physiol. 253:C514.PubMedGoogle Scholar
  11. Mauduit, P., Zoukhri, D. and Rossignol, B., 1989, Direct activation of a protein kinase activity from rat lacrimal gland by PMA in a phospholipid-free system, FEBS Lett. 252:5.PubMedCrossRefGoogle Scholar
  12. Takai, Y., Kishimoto, U., mori, T. and Nishizuka, Y., 1979, Unsaturated diacylglycerol as a possible messenger for the activation of calcium-activated, phospholipid-dependent protein kinase system, Biochem. Biophys. Res. Commun. 91:1218.PubMedCrossRefGoogle Scholar
  13. Zoukhri, D., Pelosin, J.M., Mauduit, P., Chambaz, E., Sergheraert, C. and Rossignol, B., 1992, The rat lacrimal gland expresses the α isoform of PKC: further evidence for the PMA-activated and phospholipid-independent protein kinase activity, Cell. Signal. 4:111.PubMedCrossRefGoogle Scholar
  14. Zoukhri, D., Sergheraert, C. and Rossignol, B., 1993, Phorbol ester-stimulated exocytosis in lacrimal gland: PKC might not be the sole effector, Am. J. Physiol. In press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Driss Zoukhri
    • 1
  • Philippe Mauduit
    • 1
  • Bernard Rossignol
    • 1
  1. 1.Biochimie des Transports CellulairesUniversite Paris XIOrsay CedexFrance

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