Skip to main content
SpringerLink
Log in

Subcellular Organization of Ion Transporters in Lacrimal Acinar Cells: Secretagogue-Induced Dynamics

  • Chapter
Lacrimal Gland, Tear Film, and Dry Eye Syndromes

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 350))

Abstract

The electrolyte-driven secretion of water is one of the major functions of the lacrimal gland, and impairment of this function is an obvious cause of dry eyes. As reviewed elsewhere (Mircheff, 1986, 1989), Alexander et al. showed in 1972 that the lacrimal gland produces fluid in two stages, secretion of a NaCl-rich solution in the acini, and secretion of a KCl-rich solution in the ducts. Studies of ductal transport have begun only recently (Saito, this volume). In contrast, modern concepts of acinar secretory mechanisms began to emerge more than ten years ago, when Dartt and coworkers (1981) presented the first evidence that the lacrimal glands conform to the principles of epithelial electrolyte secretion formulated by Silva and coworkers (1977),

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 299.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 379.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 379.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alexander, J.H., van Lennep, E.W., and Young, J.A., 1972, Water and electrolyte by the exorbital gland of the rat studied by micropuncture and microcatheterization techniques, Pfluegers Arch. 337:299.

    Article  CAS  Google Scholar 

  • Azuma, K.K., Bradley, M.E., Wood, R.L., McDonough, A.A., and Mircheff, A.K., 1990, Subcellular distribution of Na,K-ATPase in rat exorbital lacrimal gland, J. Cell Biol. 190a.

    Google Scholar 

  • Bradley, M.E., Lambert, R.W., Lambert, R.W., Lee, L.M., and Mircheff, A.K., 1992, Isolation and subcellular fractionation analysis of acini from rabbit lacrimal glands, Invest. Ophthalmol. Vis. Sci. 33:2951.

    PubMed  CAS  Google Scholar 

  • Bradley, M.E., Lambert, R.W., and Mircheff, A.K., 1993, Isolation and identification of plasma membrane populations. Meth. Enzymol, in press.

    Google Scholar 

  • Bradley, M.E., Peters, C.L., Lambert, R.W., Yiu, S.C., and Mircheff, A.K., 1990, Subcellular distribution of muscarinic acetylcholine receptors in rat exorbital lacrimal gland, Invest. Ophthalmol. Vis. Sci. 31:977.

    PubMed  CAS  Google Scholar 

  • Dartt, D.A., Møller, M., and Poulsen, J.H., 1981, Lacrimal gland electrolyte and water secretion in the rabbit: Localization and role of (Na++K+)-activated ATPase, J. Physiol. 321:557.

    PubMed  CAS  Google Scholar 

  • Fullard, R.J., and Tucker, D.L., 1991, Changes in human tear protein levels with progressively increasing stimulus, Invest. Ophthalmol. Vis. Sci. 32:2290.

    PubMed  CAS  Google Scholar 

  • Golchini, K., Lambert, R.W., Ghadishah, E., Yasharpour, F., Gierow, J.P., and Mircheff, A.K., 1991, Sodium-proton exchange in rabbit lacrimal acinar cells characterized with a pHi-sensitive dye, Invest. Ophthalmol. Vis. Sci. 32s:726.

    Google Scholar 

  • Lambert, R.W., Bradley, M.E., and Mircheff, A.K., 1988, Cl−/HCO3 − antiporters in rat lacrimal gland, Am. J. Physiol. 257:G637.

    Google Scholar 

  • Lambert, R.W., Bradley, M.E., and Mircheff, A.K., 1991, pH-sensitive anion exchanger in rat lacrimal acinar cells, Am. J. Physiol. 260:G517.

    PubMed  CAS  Google Scholar 

  • Lambert, R.W., Maves, C.A., Gierow, J.P. Wood, R.L., and Mircheff, A.K., 1993, Plasma membrane internalization and recycling in rabbit lacrimal acinar cells, Invest. Ophthalmol. Vis. Sci. 34: in press.

    Google Scholar 

  • Lambert, R.W., Maves, C.A., and Mircheff, A.K., 1991, Cholinergic stimulation induces activation of Na/H antiporters and recruitment of Na,K-ATPase in rabbit lacrimal acinar cells, Invest. Ophthalmol. Vis. Sci. 32S:728.

    Google Scholar 

  • Marty, A., Tan, Y.P., and Trautmann, A., 1984, Three types of calcium-dependent channels in rat lacrimal glands. J. Physiol. 357:293.

    PubMed  CAS  Google Scholar 

  • Maves, C.A., Rismondo, V., and Mircheff, A.K., 1992, Chronic simulation of rabbit lacrimal acinar cells decreases intracellular pools of Na,K-ATPase and other surface enzymes, Invest Ophthalmol. Vis. Sci. 33s: 1289.

    Google Scholar 

  • Mircheff, A.K., 1986, Comprehensive subcellular fractionation of rat exorbital gland: An approach to studying lacrimal gland fluid formation, in: “The Preocular Tear Film,” F.J. Holly, ed., Dry Eye Institute, Lubbock.

    Google Scholar 

  • Mircheff, A.K., 1989, Lacrimal fluid and electrolyte secretion: A review, Current Eye Res. 8:607.

    Article  CAS  Google Scholar 

  • Mircheff, A.K., Ingham, C.E., Lambert, R.W., Hales, K.L., Hensley, C.B., and Yiu, S.C., 1987, Na/H antiporter in lacrimal acinar cell basal-lateral membranes, Invest. Ophthalmol. Vis. Sci. 28:1726.

    PubMed  CAS  Google Scholar 

  • Mircheff, A.K., Lu, C.C., and Conteas, CN., 1983, Resolution of apical and basal-lateral membrane populations from rat exorbital lacrimal gland, Am. J. Physiol. 245:G661.

    PubMed  CAS  Google Scholar 

  • Petersen, O.H., 1986, Calcium-activated potassium channels and fluid secretion by exocrine glands. Am. J. Physiol. 251:G1.

    PubMed  CAS  Google Scholar 

  • Saito, Y., Ozawa, T., Hayashi, H., and Nishiyama, A., 1985, Acetylcholine-induced change in intracellular Cl− activity of the mouse lacrimal acinar cells, Pfluegers Arch. 405: 108.

    Article  CAS  Google Scholar 

  • Saito, Y., Ozawa, T., and Nishiyama, A., 1988, Intracellular pH regulation in the mouse lacrimal gland acinar cells, J. Membrane Biol. 101:73.

    Article  CAS  Google Scholar 

  • Silva, P., Stoff, J., Field, M., Fine, L, Forrest, J.N., and Epstein, F.H., 1977, Mechanism of active chloride secretion by shark rectal gland: role of Na+,K+-ATPase in chloride transport, Am. J. Physiol. 233:F298.

    PubMed  CAS  Google Scholar 

  • Wood, R.L., and Mircheff, A.K., 1986, Apical and basal-lateral Na,K-ATPase in rat exorbital gland, Invest. Ophthalmol. Vis. Sci. 27: 1293.

    PubMed  CAS  Google Scholar 

  • Yiu, S.C., Lambert, R.W., Bradley, M.E., Ingham, C.E., Hales, K.L., Wood, R.L., and Mircheff, A.K., 1988, Stimulation-associated redistribution of Na,K-ATPase in rat lacrimal gland. J. Membrane Biol. 102:185.

    Article  CAS  Google Scholar 

  • Yiu, S.C., Lambert, R.W., Tortoriello, P.J., and Mircheff, A.K., 1991, Secretagogue-induced redistributions of Na,K-ATPase in rat lacrimal acini. Invest. Ophthalmol. Vis. Sci. 32:2976.

    PubMed  CAS  Google Scholar 

  • Yiu, S.C., Wood, R.L., and Mircheff, A.K., 1990, Analytic fractionation of acini from rat lacrimal gland, Invest. Ophthalmol. Vis. Sci. 31:2437.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology and Biophysics Ophthalmology, University of Southern California School of Medicine, Los Angeles, CA, 90033, USA

    Austin K. Mircheff, Ross W. Lambert, Robert W. Lambert, Carol A. Maves, J. Peter Gierow & Richard L. Wood

  2. Department of Anatomy and Cell Biology, University of Southern California School of Medicine, Los Angeles, CA, 90033, USA

    Austin K. Mircheff, Ross W. Lambert, Robert W. Lambert, Carol A. Maves, J. Peter Gierow & Richard L. Wood

Authors
  1. Austin K. Mircheff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ross W. Lambert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert W. Lambert
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Carol A. Maves
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Peter Gierow
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Richard L. Wood
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Schepens Eye Research Institute and Harvard Medical School, Boston, Massachusetts, USA

    David A. Sullivan

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer Science+Business Media New York

About this chapter

Cite this chapter

Mircheff, A.K., Lambert, R.W., Lambert, R.W., Maves, C.A., Gierow, J.P., Wood, R.L. (1994). Subcellular Organization of Ion Transporters in Lacrimal Acinar Cells: Secretagogue-Induced Dynamics. In: Sullivan, D.A. (eds) Lacrimal Gland, Tear Film, and Dry Eye Syndromes. Advances in Experimental Medicine and Biology, vol 350. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2417-5_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-2417-5_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6025-4

  • Online ISBN: 978-1-4615-2417-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation