Pitfalls in the Laboratory Diagnosis of Lyme Borreliosis

  • Sally J. Cutler
  • David J. M. Wright
Part of the NATO ASI Series book series (NSSA, volume 260)

Abstract

Over a decade has passed since the identification of Borrelia burgdorferi as the causative agent of Lyme borreliosis, yet laboratory diagnosis of Lyme borreliosis remains problematic. Conventional bacteriological methods of microscopy and culture have been used with only limited success. Numbers of spirochaetes are too low for diagnosis by direct microscopic examination and pseudospirochaetes may falsely be reported as positive. Results of culture for Borrelia in the United Kingdom have been disappointing, with only one isolate obtained to date in our laboratory.

Keywords

Dermatol Sera Plague 

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References

  1. 1.
    S.J. Cutler, and D.J.M. Wright, Comparison of immunofluorescence and enzyme linked immunosorbent assays for diagnosing Lyme disease, J Clin Pathol. 42: 869–871 (1989).PubMedCrossRefGoogle Scholar
  2. 2.
    K.Hansen, P. Hindersson, and N.S. Pedersen, Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease, J Clin Microbiol. 26: 338–346 (1988).Google Scholar
  3. 3.
    L.A. Magnarelli, J.F. Anderson, and A.G. Barbour, Enzyme-linked immunosorbent assays for Lyme disease: reactivity of subunits of Borrelia burgdorferi, J Infect Dis. 159: 43–49 (1989).PubMedCrossRefGoogle Scholar
  4. 4.
    V.P. Berardi, K.E. Weeks, and A.C. Steere, Serodiagnosis of early Lyme disease: analysis of IgM and IgG responses using an antibody-capture enzyme immunoassay, J Infect Dis. 158: 754–760 (1988).PubMedCrossRefGoogle Scholar
  5. 5.
    B.W. Berger, A.B. MacDonald, and J.L.Benach, Use of an autologous antigen in the serologic testing of patients with erythema migrans of Lyme disease, J Am Acad Dermatol. 18: 1243–1246 (1988).PubMedCrossRefGoogle Scholar
  6. 6.
    M.G. Golightly, and A.L. Viciana, ELISA and immunoblots in the diagnosis of Lyme borreliosis: sensitivities and sources of false-positive results, in: Lyme disease molecular and immunologic approaches. Current Communications in Cell & Molecular Biology, 6: 283–297, S.E. Schutzer, ed., Cold Spring Harbor Laboratory Press (1992).Google Scholar
  7. 7.
    R.L. Grodzicki, and A.C. Steere, Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease, J Infect Dis. 157: 790–797 (1988).PubMedCrossRefGoogle Scholar
  8. 8.
    S.J.Cutler, D.J.M. Wright, and V. H. Luckhurst, Simplified method for the interpretation of immunoblots for Lyme borreliosis, FEMS Immunol & Med Microbiol. 6: 281–285 (1993).CrossRefGoogle Scholar
  9. 9.
    K. Hansen, J.M. Bangsborg, H. Fjorduang,N.S. Pedersen, and P. Hindersson Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60 kilodalton antigen common to a wide range of bacteria, Infect Immun. 56: 2047–2053 (1988).PubMedGoogle Scholar
  10. 10.
    M.M. Carreiro, D.C. Laux, and D.R. Nelson, Characterization of the heat shock response and identification of heat shock protein antigens of Borrelia burgdorferi, Infect Immun, 58: 2186–2191 (1990).PubMedGoogle Scholar
  11. 11.
    G. Baranton, D. Postic, I. Saint Girons et al. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis, Int J Syst Bacteriol. 42: 378–383 (1992).PubMedCrossRefGoogle Scholar
  12. 14.
    S. Baig, T. Olsson, and H. Link Predominance of Borrelia burgdorferi specific B cells in cerebrospinal fluid in neuroborreliosis, Lancet 334: 71–74 (1989).Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Sally J. Cutler
    • 1
  • David J. M. Wright
    • 1
  1. 1.Department of Medical MicrobiologyCharing Cross HospitalLondonEngland

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