Abstract
We cloned a 3.8 kb EcoRI fragment from the 29 kb circular plasmid of the Borrelia burgdorferi sensu stricto type strain B31, which hybridized to several additional restriction fragments of the 29 kb circular and the 50 kb linear plasmid of this strain (1.4 kb, 6.4 kb, 7.0 kb EcoRI and 4.5 kb HindIII, respectively). Three of the latter fragments were also cloned and their crosshybridizing segments identified on subclones.
Partial DNA sequence analysis of three fragments disclosed 5′ regions of putative open reading frames longer than 600 bp displaying 85–95% homology among each other.
Homologies on linear and circular plasmids of isolates of B. burgdorferi sensu stricto, B. garinii and the genospecies VS461 were detected as well, but none on B. coriaceae, B. hermsii, B. parkeri and B. turicatae genomic DNA. Thus the repeated DNA seems to be specific for B. burgdorferi sensu lato and therefore may be useful in nucleic acid based diagnosis of Lyme disease.
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Zückert, W.R., Filipuzzi-Jenny, E., Meister-Turner, J., Stålhammar-Carlemalm, M., Meyer, J. (1994). Repeated DNA Sequences on Circular and Linear Plasmids of Borrelia Burgdorferi Sensu Lato. In: Axford, J.S., Rees, D.H.E. (eds) Lyme Borreliosis. NATO ASI Series, vol 260. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2415-1_36
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DOI: https://doi.org/10.1007/978-1-4615-2415-1_36
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