Mediation of H2O2-induced vascular relaxation by endothelium-derived relaxing factor

  • Lalita Bharadwaj
  • Kailash Prasad
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 15)

Abstract

We investigated the effects of H2O2 generated by glucose (G) and glucose oxidase (GO) on the isolated rabbit aorta suspended in Krebs-Ringer solution. H2O2 produced contraction in small concentration and relaxation followed by contraction in large concentration. Contraction produced by large concentration was smaller than that produced by small concentration of H2O2. Relaxation was prevented by deendothelialization or NG-monomethyl-L-arginine, an inhibitor of nitric oxide synthesis. These results suggest that H2O2 in large concentrations produces relaxation followed by contraction, and that the relaxation is endothelium-dependent and is mediated by nitric oxide, an endothelium-derived relaxing factor.

Key words

hydrogen peroxide rabbit aorta NG-monomethyl-L-arginine deendothelialization glucose oxidase 

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Copyright information

© Springer Science+Business Media Dordrecht 1995

Authors and Affiliations

  • Lalita Bharadwaj
    • 1
  • Kailash Prasad
    • 1
  1. 1.Department of Physiology, College of MedicineUniversity of SaskatchewanSaskatoonCanada

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