Umbilical Cord Blood Stem Cell Transplantation

  • John E. Wagner
Part of the Cancer Treatment and Research book series (CTAR, volume 76)


Early successes with the transplantation of umbilical cord blood have prompted considerable investigation of this stem cell source. Numerous laboratory investigators have subsequently confirmed the high frequency of primitive hematopoietic progenitors as well as initiated a description of the functional capacities of the neonatal immune system. As a result of these clinical and laboratory observations, the large-scale banking of umbilical cord blood for future transplantation has been initiated worldwide.


Human Leukocyte Antigen Umbilical Cord Blood Human Umbilical Cord Blood Hematopoietic Recovery Cord Blood Banking 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Moore MAS, Metcalf D: Ontogeny of the haematopoietic system: Yolk sac origin in vivo and in vitro colony forming cells in the developing mouse embryo. Br J Haematol 18: 279–285, 1970.PubMedCrossRefGoogle Scholar
  2. 2.
    Nathan DG, Housman DE, Clarke BJ: The anatomy and physiology of hematopoiesis. In Nathan DG, Oski FA (eds): Hematology of Infancy and Childhood. Philadelphia: WB Saunders, 1981, pp 144–167.Google Scholar
  3. 3.
    Knudtzon S: In vitro growth of granulocyte colonies from circulating cells in human cord blood. Blood 43:357–361, 1974.PubMedGoogle Scholar
  4. 4.
    Gabutti V, Foa R, Mussa F, Aglietta M: Behavior of human hematopoietc stem cells in cord and neonatal blood. Haematologica 4:60, 1975.Google Scholar
  5. 5.
    Broxmeyer HE, Douglas GW, Hangoc G, Cooper S, Bard J, English D, Arny M, Thomas L, Boyse EA: Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proc Natl Acad Sci USA 86:3828–3832, 1989.PubMedCrossRefGoogle Scholar
  6. 6.
    Broxmeyer HE, Hangoc G, Cooper S, Riberiro RC, Graves V, Yoder M, Wagner J, Vadhad-Raj S, Rubinstein P, Broun ER: Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation of adults. Proc Natl Acad Sci USA 89:4109–4113, 1992.PubMedCrossRefGoogle Scholar
  7. 7.
    Wagner JE, Kernan NA, Broxmeyer HE, Gluckman E: Allogeneic umbilical cord blood transplantation: Report of results in 26 patients. Bood 82:86, 1993.Google Scholar
  8. 8.
    Wagner JE: Umbilical cord blood transplantation: Overview of the clinical experience. Blood Cells, 1994, in press.Google Scholar
  9. 9.
    Broxmeyer HE, Kurtzberg J, Gluckman E, Auerbach AD, Douglas G, Cooper S, Falkenberg JHF, Bard J, Boyse EA: Umbilical cord blood hematopoietic stem and repopulating cells in human clinical transplantation. Blood Cells 17:313–329, 1991.PubMedGoogle Scholar
  10. 10.
    Gluckman E, Broxmeyer HE, Auerbach AD, Friedman H, Douglas GW, Devergie A, Esperou H, Thierry D, Socie G, Lehn P, Cooper S, English D, Kurtzberg J, Bard J, Boyse EA: Hematopoietic reconstitution in a patient with Fanconi’s anemia by means of umbilical cord blood from an HLA-identical sibling. N Engl J Med 321:1174–1178, 1989.PubMedCrossRefGoogle Scholar
  11. 11.
    Vilmer E, Sterkers G, Rahimy C, Elion J, Broyart A, Lescoeur B, Gerota J, Blot P: HLA-mismatched cord blood transplantation in a patient with advanced leukemia. Transplantation 53:1155–1157, 1992.PubMedCrossRefGoogle Scholar
  12. 12.
    Wagner JE, Broxmeyer HE, Byrd RL, Zehnbauer B, Schmeckpeper B, Shah N, Griffin C, Emanuel PD, Zuckerman KS, Cooper S, Carow C, Bias W, Santos GW: Transplantation of umbilical cord blood after myeloablative therapy: Analysis of engraftment. Blood 79:1874–1881, 1992.PubMedGoogle Scholar
  13. 13.
    Vowels MR, Lam-PO-Tang R, Berdoukas V, Ford D, Thierry D, Purtilo D, Gluckman E: Brief report: Correction of X-linked lymphoproliferative disease by transplantation of cord-blood stem cells. N Engl J Med 329:1623–1625, 1993.PubMedCrossRefGoogle Scholar
  14. 14.
    Bogdanic V, Nemet D, Kastelan A, Latin V, Petrovecki M, Brkljacic-Surlakovic L, Kerki-Brkljacic V, Aurer I, Konja J, Mrsic M, Kalenic S, Labar B: Umbilical cord blood transplantation in a patient with Philadelphia-chromosome positive chronic myeloid leukemia. Transplantation 56:477–479, 1993.PubMedCrossRefGoogle Scholar
  15. 15.
    Wagner JE, Broxmeyer HE, Cooper S: Umbilical cord and placental blood hematopoietic stem cells: Collection, cryopreservation and storage. J Hematother 1:167–173, 1992.PubMedCrossRefGoogle Scholar
  16. 16.
    Turner CW, Luzins J, Hutcheson C: A modified harvest technique for cord blood hematopoietic stem cells. Bone Marrow Transplant 10:89–91, 1992.PubMedGoogle Scholar
  17. 17.
    Harris DT, Schumacher MJ, Rychlik S, Booth A, Acevedo A, Rubinstein P, Bard J, Boyse EA: Collection, separation and cryopreservation of umbilical cord blood for use in transplantation. Bone Marrow Transplant 13:135–143, 1994.PubMedGoogle Scholar
  18. 18.
    Bertolini F, Lazzari L, Lauri E, Corsini C, Castelli C, Gorini F, Sirchia G: A comparative study of different procedures for the collection and banking of umbilical cord blood. J Hematother 4:29–38, 1995.PubMedCrossRefGoogle Scholar
  19. 19.
    Newton I, Charbord P, Schaal JP, Herve P: Toward cord blood banking: Density-separation and cryopreservation of cord blood progenitors. Exp Hematol 21:671–674, 1993.PubMedGoogle Scholar
  20. 20.
    Nagler A, Peacock M, Tantoco M, Lamons D, Okarma TB, Okrongly DA: Separation of hematopoietic progenitor cells from human umbilical cord blood. J Hematother 2:243–245, 1993.PubMedCrossRefGoogle Scholar
  21. 21.
    Rubinstein P, Rosenfield RE, Adamson JW, Stevens CE: Review: Stored placental blood for unrelated bone marrow reconstitution. Blood 81:1679–1690, 1993.PubMedGoogle Scholar
  22. 22.
    Gluckman E, Wagner J, Hows J, Kernan N, Bradley B, Broxmeyer HE: Cord blood banking for hematopoietic stem cell transplantation: An international cord blood transplant registry. Bone Marrow Transplant 11:199–200, 1993.PubMedGoogle Scholar
  23. 23.
    Kurtzberg J, Casey J, Miller S, Olsen J, Ciocci G, Graham MJ, Stevens C, Rubinstein P: Alternative transplantation of umbilical cord blood cells in pediatric patients. Exp Hematol 22:689, 1994.Google Scholar
  24. 24.
    Oski FA: The Hematological aspects of the maternal-fetal relationship. In Oski FA, Naiman JL (eds): Hematologic Problems in the Newborn. Philadelphia: WB Saunders, 1982, pp 32–55.Google Scholar
  25. 25.
    Socié G, Gluckman E, Carosella E, Brossard Y, Lafon C, Brison O: Search for maternal cells in human umbilical cord blood by polymerase chain reaction by amplification of two minisatellite sequences. Blood 83:340–344, 1994.PubMedGoogle Scholar
  26. 26.
    Saeland S, ouvert V, Caux C, Pandrau D, Favre C, Valle A, Durand I, Charbord P, de Vries J, Banchereau J: Distribution of surface-membrane molecules on bone marrow and cord blood CD34+ hematopoietic cells. Exp Hematol 20:24–33, 1992.PubMedGoogle Scholar
  27. 27.
    Verfaillie C, Blakolmer K, McGlave P: Purified primitive human hematopoietic progenitor cells with long-term in vitro repopulating capacity adhere selectively to irradiated bone marrow stroma. J Exp Med 172:509, 1990.PubMedCrossRefGoogle Scholar
  28. 28.
    Srour EF, Brandt JE, Briddell RA, Grigsby S, Leemhuis T, Hoffman R: Long-term generation and expansion of human primitive hematopoietic progenitor cells in vitro. Blood 81:661–669, 1993.PubMedGoogle Scholar
  29. 29.
    Traycoff CM, Abboud MR, Laver J, Brandt JE, Hoffman R, Law P, Ishizawa L, Srour EF: Evaluation of the in vitro behavior of phenotypically defined populations of umbilical cord blood hematopoietic progenitor cells. Exp Hematol 22:215–222, 1994.PubMedGoogle Scholar
  30. 30.
    Dugan MJ, Han CS, McGlave PB: Committed and primitive progenitor content of umbilical cord blood CD34+ subpopulations. Exp Hematol 22:791, 1994.Google Scholar
  31. 31.
    Moore MAS: Ex vivo expansion and gene therapy using cord blood CD34+ cells. J Hematother 2:221–224, 1993.PubMedCrossRefGoogle Scholar
  32. 32.
    Cardoso AA, Li ML, Hatzfeld A, Brown EL, Levesque JP, Sookdeo H, Batard P, Clark SC, Hatzfeld J: Release from quiescence of CD34+ CD38— human umbilical cord blood cells reveals their potentiality to engraft adults. Proc Natl Acad Sci USA 90:8707–8712, 1993.PubMedCrossRefGoogle Scholar
  33. 33.
    Verfaillie CM: Direct contact between primitive hematopoietic progenitors and bone marrow stroma is not required for long term in vitro hematopoiesis. Blood 79:2821–2826, 1992.PubMedGoogle Scholar
  34. 34.
    Verfaillie CM: Soluble factor(s) produced by human bone marrow stroma increase cytokine induces proliferation and maturation of primitive hematopoietic progenitors while preventing their terminal differentiation. Blood 82:2045–2054, 1993.PubMedGoogle Scholar
  35. 35.
    Verfaillie CM: MlP-la combined with IL3 conserves primitive human LTBMC-IC for at least 8 weeks in ex vivo’ stroma non-contact’ cultures. J Exp Med 179:643–649, 1994.PubMedCrossRefGoogle Scholar
  36. 36.
    Han CS, Dugan MJ, Verfaillie CM, Wagner JE, McGlave PB: In vitro expansion of umbilical cord blood committed and primitive progenitors. Exp Hematol 22:723a, 1994.Google Scholar
  37. 37.
    Rainaut M, Pagniez M, Hercend T, Dafos F, Forestier F: Characterization of mononuclear cell subpopulations in normal fetal peripheral blood. Human Immunol 18:331–337, 1987.CrossRefGoogle Scholar
  38. 38.
    Hannet I, Erkeller-Yuksel F, Lydyard P, Deneys V, DeBruyere M: Developmental and maturational changes in human blood lymphocyte subpopulations. Immunol Today 13:215–218, 1992.PubMedCrossRefGoogle Scholar
  39. 39.
    Clement LT, Vink PE, Bradley GE: Novel immunoregulatory functions of phenotypically distinct subpopulations of CD4+ cells in the human neonate. J Immunol 145:102–108, 1990.PubMedGoogle Scholar
  40. 40.
    Roncarolo MG, Bigler M, Ciuti E, Martino S, Tovo P-A: Immune responses by cord blood cells. Blood Cells, 1994, in press.Google Scholar
  41. 41.
    Risdon G, Gaddy J, Stehman FB, Broxmeyer HE: Proliferative and cytotoxic responses of human cord blood T lymphocytes following allogeneic stimulation. Cell Immunol 154: 14–24, 1994.PubMedCrossRefGoogle Scholar
  42. 42.
    Boyse EA, Broxmeyer HE, Douglas AW: Preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood. U.S. Patent number 5,004,681 issued April 2, 1992; assigned to Biocyte Corporation.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • John E. Wagner

There are no affiliations available

Personalised recommendations