Abstract
In recent years it became clear that the regulation of cell function involves another important controlling parameter, i.e. the cellular hydration state. Cellular hydration can change within minutes under the influence of hormones, nutrient supply and oxidative stress and such a short-term modulation of cell volume within a narrow range acts per se as a potent signal which modifies cellular metabolism and gene expression. The interaction between cellular hydration and cell function was most extensively studied in liver cells, but evidence is increasing that modulation of cell function in response to alterations of cellular hydration is a general mechanism. Disturbances of cellular hydration as a pathogenetic factor for the development of hyperammonemia and hepatic encephalopathy have not yet been considered, but very recent evidence suggests interesting aspects for the understanding of hyperammonemic states. Thus, the article will briefly review the current state of knowledge regarding the interaction between hepatocellular volume and liver function and then discuss some pathophysiological conditions in which disturbances of cellular volume could be of pathogenetic relevance.
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References
Kilberg M. & Häussinger, D., eds. (1992) Mammalian amino acid transport: mechanisms and control. Plenum Press New York.
Häussinger D. & Lang, F. (1992) Cell volume and hormone action. Trends Pharmacol.Sci. 13:371–373.
Hallbrucker, C., Ritter, M., Lang, F., Gerok W. & Häussinger, D. (1993) Hydroperoxide metabolism in rat liver. K+ channel opening, cell volume changes and eicosanoid formation. Eur. J. Biochem. 211:449–458.
Saha, N., Schreiber, R., vom Dahl, S., Lang, F., Gerok, W. & Häussinger, D. (1993) Endogenous hydroperoxide formation, cell volume and cellular K+ balance in perfused rat liver. Biochem J. 296:701–707.
Häussinger, D. & Lang, F. (1991) Cell volume in the regulation of hepatic function: a new mechanism for metabolic control. Biochim. Biophys. Acta 1071:331–350.
Mortimore, G.E. & Pösö, A.R. (1987) Intracellular protein catabolism and its control during nutrient deprivation and supply. Ann. Rev. Nutr. 7:539–564.
Seglen, P.O. & Gordon, P.B. (1984) Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes. J. Cell. Biol. 99:435–444.
Häussinger, D., Hallbrucker, C., vom Dahl, S., Decker, S., Schweizer, U., Lang, F. Gerok, W.(1991) Cell volume is a major determinant of proteolysis control in liver. FEBS Lett. 283:70–72.
Vom Dahl, S., Hallbrucker, C, Lang, F., Gerok, W. & Häussinger, D. (1991a) Regulation of liver cell volume and proteolysis by glucagon and insulin. Biochem. J. 278:771–777.
Häussinger, D., Stoll, B., vom Dahl, S., Theodoropoulos, P.A., Markogiannakis, E., Gravanis, A.,Lang, F. & Stournaras, C. (1994) Microtubule stabilization and induction of tubulin mRNA by cell swelling in isolated rat hepatocytes. Biochem. Cell. Biol. 72:12–19.
Völkl, H., Friedrich, F., Häussinger, D. & Lang, F. (1993) Effect of cell volume on acridine orange fluorescence in hepatocytes. Biochem. J. 295:11–14.
Stoll, B., Gerok, W., Lang, F. & Häussinger, D. (1992) Liver cell volume and protein synthesis.Biochem. J. 287:217–222.
Theodoropoulos, T., Stournaras, C., Stoll B., Markogiannakis, E., Lang, F., Gravani, A. & Häussinger, D. (1992) Hepatocyte swelling leads to rapid decrease of G-/total actin ratio and increases actin mRNA levels. FEBS Lett. 311:241–245.
Häussinger, D., Saha, N., Hallbrucker, C., Lang, F. & Gerok, W. (1993) Involvement of microtubules in the swelling-induced stimulation of transcellular taurocholate transport in perfused rat liver. Biochem. J. 291:355–360.
Schreiber, R., Stoll, B., Lang, F. & Häussinger, D. (1994) Effects of anisotonicity on intracellular pH in isolated rat hepatocytes as assessed by BCECF and FITC-dextran fluorescence. Biochem. J. in press.
Tager, J.M., Aerts, J.M.F.G., Oude-Elferink, R.J.A., Groen, A.K., Holle mans, M. & Schram,A.W. (1988) pH regulation of intracellular membrane flow. In: pH Homeostasis (Häussinger, D., Ed.) pp.123–162, Academic Press London.
Häussinger, D., Hallbrucker, C., Saha, N., Lang, F. & Gerok, W. (1992) Cell volume and bile acid excretion. Biochem. J. 288:681–689.
Finkenzeller, G., Newsome, W.P., Lang, F. & Häussinger, D. (1994) Increase of c-jun mRNA upon hypoosmotic cell swelling of rat hepatoma cells. FEBS Lett. 340:163–166.
Tohyama, Y., Kameji, T. & Hayashi, S. (1991) Mechanisms of dramatic fluctuations of ornithine decarboxylase activity upon tonicity changes in primary cultured rat hepatocytes. Eur. J. Biochem. 202:1327–1331.
Newsome, W.P., Warskulat, U., Noe, B., Wettstein, M., Stoll, B., Gerok,W. & Häussinger, D.(1994) Modulation of phosphoenolpyruvate carboxykinase mRNA levels by the hepatocellular hydration state. Biochem. J. in press.
Gazzola, G.C., Dall’Asta, V., Nucci, F.A., Rossi, P.A., Bussolati, O., Hoffmann, E.K. & Guidotti,G.G. (1991) Role of amino acid transport system A in the control of cell volume in cultured human fibroblasts. Cell. Physiol. Biochem. 1:131–142.
Lang, F., Ritter, M., Wöll, E., Weiss, H., Häussinger, D., Maly, K. & Grunicke, H. (1992) Altered cell volume regulation in ras oncogene expressing NIH fibroblasts. Pflügers Arch. Physiol. 420:424–427.
Bianchini, L. & Grinstein, S. (1993) Regulation of volume-modulating ion transport systems by growth promoters. In: Interaction of cell volume and cell function (Lang, F. & Häussinger, D., eds.) pp.249–277, Springer Verlag Heidelberg.
Grinstein S., Furuya, W. & Bianchini, L. (1992) Protein kinases, phosphatases, and the control of cell volume. News Physiol. Sci. 7:232–237.
Halestrap, A.P. (1993) The regulation of organelle function through changes of their volume. In:Interactions of cell volume and cell function (Lang, F. & Häussinger, D., eds.) pp.279–307, Springer Verlag Heidelberg.
Hoffmann, E.K., Simonsen, L.O. & Lambert, I.H. (1993) Cell volume regulation: intracellular transmission. In: Interactions of cell volume and cell function (Lang, F. & Häussinger,, eds) pp.187–248, Springer Verlag Heidelberg.
Lang, F. & Häussinger, D., eds. (1993) Interaction of cell volume and cell function. Springer Verlag Heidelberg.
McCarty, N.A. & O’Neil, R.G. (1992) Calcium signalling in cell volume regulation. Physiol. Rev.72:1037–1061.
Minton, A.P., Colclasure, G.C. & Parker, J.C. (1992) Model for the role of macromolecular crowding in regulation of cellular volume. Proc. Nat. Acad. Sci. 89:10504–10506.
Häussinger, D., Roth, E., Lang, F. & Gerok, W. (1993) Cellular hydration state: an important determinant of protein catabolism in health and disease. Lancet 341:1330–1332.
Ferenci, P., Püspök, A., Steindl, P. (1992) Current concepts in the pathogenesis of hepatic encephalopathy. Eur. J. Clin. Invest. 22:573–581.
Lockwood, A.H. (1992) Hepatic encephalopathy. Butterworth-Heinemann Boston.
Norenberg, M.D., Neary, J.T., Bender, A.S. & Dombro, R.S. (1992) Hepatic encephalopathy: a disorder in glial-neuronal communication. Progress Brain Res. 94:261–269.
Martinez-Hernandez, A., Bell, K.P. & Norenberg, M.D. (1977) Glutamine synthetase: glial localization in brain. Science 195:1356–1358.
Kimeiberg, H.K., O Connor, E.R. & Kettenmann, H. (1993) Effects of swelling on glial cell function. In: Interactions cell volume and cell function (Lang, F., Häussinger, D., eds,) pp.158–186, Springer Verlag Heidelberg.
Lien, Y.H.H., Shapiro, J.L. & Chan, L. (1990) Effects of hypernatremia on organic brain osmoles.J. Clin. Invest. 85:1427–1435.
Murphy S. ed. (1993) Astrocytes-Pharmacology and function. Academic Press San Diego.
Häussinger, D., Laubenberger, J., vom Dahl, S., Ernst, T., Bayer, S., Langer, M., Gerok, W. & Hennig, J. (1994). Proton Magnetic Resonance Spectroscopic Studies on Human Brain Myoinositol in Hypoosmolarity and Hepatic Encephalopathy. Gastroenterology, in press.
Sterns, R.H., Baer, J., Ebersol, S., Thomas, D., Lohr, J.W., Kamm, D.E. (1993) Organic osmolytes in acute hyponatremia. Am. J. Physiol. 264:F833–F836.
Lee, J.H., Ross, B.D. (1993) Quantitation of idiogenic osmoles in human brain. Abstr. Commun.12th Ann. Meet. Soc. MR Med. 1993; 1553.
Kreis, R., Farrow, N.A. & Ross, B.D. (1990) Diagnosis of hepatic ence phalopathy by proton magnetic resonance spectroscopy. Lancet 336:635–636.
Kreis, R., Ross, B.D., Farrow, N.A. & Ackerman, Z. (1992) Metabolic disorders of the brain in chronic hepatic encephalopathy detected with H-1 MR spectroscopy. Radiology 182:19–27.
Moats, R.A., Lien, Y.H.H., Filippi, D. & Ross, B.D. (1993) Decrease in cerebral inositols in rats and humans. Biochem. J. 295:15–18.
Norenberg, M.D., Baker, L., Norenberg, L.O.B., Blicharska, J., Bruce-Gregorius, J.H. & Neary,J.T. (1991) Ammonia-induced astrocyte swelling in primary culture. Neurochem. Res. 16:833–836.
Hawkins, R.A., Jessy, J., Mans, A.M. & De Joseph, M.R. (1993) Effect of reducing brain glutamine synthesis on metabolic symptoms of hepatic encephalopathy. J. Neurochem. 60:1000–1006.
Häussinger, D., Lang, F. Bauers, K. & Gerok, W. (1990) Interactions between glutamine metabolism and cell volume regulation in perfused rat liver. Eur. J. Biochem. 188:689–695.
Offensperger, W.B., Offensperger, S., Stoll, B. Gerok, W. & Häussinger, D. (1994) Effect of anisotonic exposure on duck hepatitis B virus replication. Hepatology, in press.
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Häussinger, D., Gerok, W. (1994). Role of the Cellular Hydration State for Cellular Function: Physiological and Pathophysiological Aspects. In: Felipo, V., Grisolia, S. (eds) Hepatic Encephalopathy, Hyperammonemia, and Ammonia Toxicity. Advances in Experimental Medicine and Biology, vol 368. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1989-8_4
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DOI: https://doi.org/10.1007/978-1-4615-1989-8_4
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