High Density Culture of Immuno-Magnetically Separated Human Mammary Luminal Cells

  • Catherine Clarke
  • Paul Monaghan
  • Michael J. O’Hare

Abstract

When the two epithelial cell types of the human breast, myoepithelial and luminal, are grown together in vitro, the myoepithelial cells come to dominate cultures because of their greater proliferative potential. However, it is the luminal cells which are of interest in many studies since they are the source of most breast cancers. In order to study luminal cells in culture, the two cell types must be separated. This has previously been achieved using flow cytometry (O&3x2019;Hare et al., 1991) with numbers limited to about 105 cells of each type. For the current study of differentiation of luminal cells, large numbers of cells were required and an immunomagnetic separation technique was developed using the MACS system (Miltenyi et al., 1990; Clarke et al., 1994). Such purified populations of luminal cells have been grown on various substrates at high density, and their structural differentiation examined by light and electron microscopy.

Keywords

Hydrocortisone Luminal Sorting Cholera 

References

  1. Clarke, C., Titley, J., Davies S. and O’Hare, M.J., 1994, An immunomagnetic separation method using superparamagnetic (MACS) beads for large-scale purification of human mammary luminal and myoepithelial cells, Epithelial Cell Biol. ,3:38.PubMedGoogle Scholar
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Catherine Clarke
    • 1
  • Paul Monaghan
    • 1
  • Michael J. O’Hare
    • 1
  1. 1.Haddow LaboratoriesInstitute of Cancer ResearchSutton, SurreyUK

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