IL-10 Inhibits the Primary Allogeneic T Cell Response to Human Peripheral Blood Dendritic Cells
Most of the immunosuppressive properties of IL-10 are related to its action on antigen presenting cells (APC). Until now, several mechanisms have been described by which IL-10 inhibits the ability of monocytes/macrophages to induce T cell activation. Indeed, IL-10 down-regulates their expression of MHC class II molecules1 as well as of ICAM-1 and B7-1 accessory molecules2,3. Moreover, the inhibition by IL-10 of IL-12 and IL-1 synthesis by monocytes was shown to be responsible for the inhibition of IFN-y production by peripheral blood mononuclear cells (PBMC)4. As far as the effects of IL-10 on dendritic cells are concerned, IL-10 was shown to inhibit the ability of mouse splenic dendritic cells to induce interferon (IFN)-y production by antigen-specific TH1 clones or alloreactive splenic T cells5. In addition, human epidermal Langerhans cells preincubated with IL-10 were found to be deficient in the triggering of a mixed lymphocyte reaction6. As a first approach to study the effects of IL-10 on human peripheral blood dendritic cells (PBDC), we analyzed the influence of IL-10 on a primary allogeneic T cell response induced by those cells.
KeywordsDendritic Cell Peripheral Blood Mononuclear Cell Mixed Lymphocyte Reaction Nonadherent Cell Accessory Molecules2
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- 1.R. de Waal Malefyt, J. Haanen, H. Spits, M.G. Roncarlo, A. te Velde, C. Figdor. K. Johnson, R. Kastelein, H. Yssel, and J.E. de Vries, Interleukin 10 and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression,.J. Exp. Med. 174:915 (1991).PubMedCrossRefGoogle Scholar