Abstract
Previous studies have shown that dendritic cells (DC) isolated from mouse lymph nodes or spleen can induce primary proliferative responses to contact sensitizers when exposed to the chemical either in vivo or in vitro 1,2 In this study we have attempted to induce primary immune responses to contact sensitizers in vitro using peripheral blood DC. Neither human nor mouse blood DC stimulated a response to picryl sulphonic acid by syngeneic lymphocytes. This contrasted with mouse lymph node or spleen DC where good primary proliferative responses to picryl sulphonic acid have been obtained. Here we have examined adhesion molecule expression on mouse lymph node and blood DC to determine whether functional variation is related to surface molecule expression.
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References
S.C.Knight, J.Krecjci, M.Malkovsky, V.Colizzi, A.Gautam & G.L.Asherson. The role of dendritic cells in the initiation of immune responses to contact sensitizers. 1. In vivo exposure to antigen Cell. Immunol. 94:427 (1985).
S.E.Macatonia, A.J.Edwards & S.C.Knight. Dendritic cells and the initiation of contact sensitivity to fluorescein isothiocyanate. Immunology 59:509 (1986).
S.E.Macatonia, S.Patterson & S.C.Knight. Primary proliferative and cytotoxic T cell responses to HTV induced in vitro by human dendritic cells. Immunology 74:399 (1991)
AJ.Stagg, B.Harding, R.A.Hughes, A.C.S.Keats & S.C.Knight. Peripheral blood and synovial T cells differ in their response to alloantigens and recall antigens presented by dendritic cells. Clin.Exp.Med. 84:72 (1991)
P..King & D.R.Katz. Human tonsillar dendritic cell-induced responses: analysis of molecular mechanisms using monoclonal antibodies. Eur.J.Immunol. 19:581 (1989)
1.Inaba & R.M.Steinman. Monoclonal antibodies to LFA-1 and CD4 inhibit the mixed leukocyte reaction after antigen-dependent clustering of dendritic cells and T lymphocytes. J.Exp.Med. 165:1403(1987).
M.Cumberbatch, I.Illingworth & I.Kimber. Antigen-bearing dendritic cells in the draining lymph nodes of contact sensitized mice: cluster formation with lymphocytes. Immunology 74:139 (1991).
M.Goebeler, J.Gutwald, J.Roth, G.Meinhardus-Hager & C.Sorg. Expression of intercellular adhesion molecule-1 in murine allergic contact dermatitis. Int.Arch.Allergy Appl. Immunol. 93:294 (1990).
A.Scheynius, R.L.Camp & E.Pure. Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1. J.Immunol. 150:655 (1993).
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© 1995 Springer Science+Business Media New York
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Hill, S., Coates, J.P., Kimber, I., Knight, S.C. (1995). Functional Dichotomy of Dendritic Cells Isolated from Blood and Lymph Nodes. In: Banchereau, J., Schmitt, D. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 378. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1971-3_44
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DOI: https://doi.org/10.1007/978-1-4615-1971-3_44
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