Establishment and Characterization of Antigen-Presenting Cell Lines (XS Series) Derived from Newborn Mouse Epidermis
Activation of naive T cells occurs primarily through antigen presentation by a distinct class of leukocytes, termed dendritic cells (DC) (reviewed in 1). Langerhans cells (LC) are a skin-specific member of this family and play a pivotal role in the induction of T cell-mediated immunity against various antigens that are present in or penetrate into skin, which include reactive chemicals (contact hypersensitivity), alloantigens (skin graft rejection), microorganisms (protective anti-infectious immunity), and tumor-associated antigens (protective antitumor immunity) (reviewed in 2). A major limitation in studying the biology of LC has been the absence of stable, long-term cell lines. To overcome this limitation, we have established a series of antigen presenting cell lines (XS series) from newborn BALB/c mouse epidermis (3,4). Here we describe the establishment and characterization of XS lines.
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