Advertisement

Establishment and Characterization of Antigen-Presenting Cell Lines (XS Series) Derived from Newborn Mouse Epidermis

  • Akira Takashima
  • Shan Xu
  • Kiyoshi Ariizumi
  • Paul R. Bergstresser
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 378)

Abstract

Activation of naive T cells occurs primarily through antigen presentation by a distinct class of leukocytes, termed dendritic cells (DC) (reviewed in 1). Langerhans cells (LC) are a skin-specific member of this family and play a pivotal role in the induction of T cell-mediated immunity against various antigens that are present in or penetrate into skin, which include reactive chemicals (contact hypersensitivity), alloantigens (skin graft rejection), microorganisms (protective anti-infectious immunity), and tumor-associated antigens (protective antitumor immunity) (reviewed in 2). A major limitation in studying the biology of LC has been the absence of stable, long-term cell lines. To overcome this limitation, we have established a series of antigen presenting cell lines (XS series) from newborn BALB/c mouse epidermis (3,4). Here we describe the establishment and characterization of XS lines.

Keywords

Splenic Dendritic Cell Dendritic Cell Line Skin Graft Rejection Fibroblastic Stromal Cell Heat Stable Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Steinman RM: The dendritic cell system and its role in immunogenicity. Ann Rev Immunol9:271–296, 1991CrossRefGoogle Scholar
  2. 2.
    StinglG, Hauser C, Wolff K: The Epidermis: An Immunologic Microenvironment,Dermatology in General Medicine. Edited by Fitzpatrick TB. New York, McGrawHill and Co., 1993, pp 172–197Google Scholar
  3. XuS, Ariizumi K, Caceres-Dittmar G, Edelbaum D, Hashimoto K, Bergstresser PR,Takashima A: Successive generation of antigen-presenting, dendritic cell linesfrom murine epidermis. J Immunol (in press).Google Scholar
  4. XuS, Ariizumi K, Edelbaum D, Bergstresser PR, Takashima A: Cytokine-dependentregulation of growth and maturation in murine epidermal dendritic cell lines.Submitted for publication.Google Scholar
  5. 5.
    ShimadaS, Caughman SW, Sharrow SO, Stephany D, Katz SI: Enhanced antigen-presentingcapacity of cultured Langerhans’ cells is associated with markedly increasedexpression of la antigen. J Immunol 139:2551–2555, 1987PubMedGoogle Scholar
  6. 6.
    LeeMG, Borkowski A, Udey MC: Regulation of expression of B7 by murine Langerhanscells: A direct relationship between B7 mRNA levels and the level of surfaceexpression of B7 by Langerhans cells. J Invest Dermatol 101:883–886, 1993PubMedCrossRefGoogle Scholar
  7. 7.
    TangA, Amagai M, Granger LG, Stanley JR, Udey MC: Adhesion of epidermal Langerhanscells to keratinocytes mediated by E-cadherin. Nature 361:82–85, 1993 8.CrossRefGoogle Scholar
  8. TangA, Udey MC: Differential sensitivity of freshly isolated and cultured murineLangerhans cells to ultraviolet B radiation and chemical fixation. Eur JImmunol 22:581–586, 1992CrossRefGoogle Scholar
  9. 9.
    HeuflerC, Topar G, Koch F, Trockenbacher B, Kampgen E, Romani N, Schuler G: Cytokine gene expression in murine epidermalcell suspensions: interleukin 1ß and macrophage inflammatory protein laare selectively expressed in Langerhans cells but are differentially regulatedin culture. J Exp Med 176:1221–1226, 1992PubMedCrossRefGoogle Scholar
  10. 10.
    MatsueH, Cruz PD,Jr., Bergstresser PR, Takashima A: Langerhans cells are the majorsource of mRNA for IL-1ß and MlP-lα among unstimulated mouse epidermal cells. JInvest Dermatol 99:537–541, 1992CrossRefGoogle Scholar
  11. 11.
    SchreiberS, Kilgus O, Payer E, Kutil R, Elbe A, Mueller C, Stingl G: Cytokine pattern ofLangerhans cells isolated from murine epidermal cell cultures. J Immunol149:3524–3534, 1992PubMedGoogle Scholar
  12. 12.
    KämpgenE, Koch F, Heufler C, Eggert A, Gill LL, Gillis S, Dower SK, Romani N, SchulerG: Understanding the dendritic cell lineage through a study of cytokinereceptors. J Exp Med 179:1767–1776, 1994PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Akira Takashima
    • 1
  • Shan Xu
    • 1
  • Kiyoshi Ariizumi
    • 1
  • Paul R. Bergstresser
    • 1
  1. 1.Department of DermatologyUniversity of Texas Southwestern Medical CenterDallasUSA

Personalised recommendations