Migration of Interleukin-6 Producing Langerhans Cells to Draining Lymph Nodes following Skin Sensitization
Epidermal Langerhans cells (LC) have potential to develop into active antigen presenting cells for T cell-dependent immune responses. It is likely that the accessory function of these cells is dependent not only on their ability to express certain costimulatory adhesion molecules such as B71, but also on their capacity to elaborate cytokines2,3. LC and/or the dendritic cells (DC) into which they mature, were considered originally not to produce cytokines. However, it is now known that cultured LC, which are in many ways analogous to the immunocompetent LC which have migrated to draining lymph nodes following skin sensitization, have the ability to synthesize and secrete interleukins 1ß and 6 (IL-ß and IL-6) in vitro 4. The production by LC of IL-lß and IL-6 is of interest as both cytokines are known to be important accessory molecules for T cell activation5 and may therefore participate in LC/T lymphocyte interactions during antigen presentation in vivo. Certainly, LC-derived IL-1ß has been reported recently to be essential for the induction of primary immune responses following skin sensitization of BALB/c mice6. Evidence that IL-6 may also be important, comes from studies in mice where the induction phase of skin sensitization was found to be associated closely with the production by draining lymph node cells of IL-67. Although T lymphocytes have the capacity to produce IL-6, proliferating T cells were found not to be the major source of this cytokine in allergen-activated lymph nodes. We chose to examine whether the DC which accumulate in draining lymph nodes following skin sensitization, and the epidermal LC from which they derive, express this cytokine.
KeywordsDendritic Cell Drain Lymph Node Skin Sensitization Primary Immune Response Drain Lymph Node Cell
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- 3.C. Heufler, G. Topar, F. Koch, B. Trockenbacher, E. Kampgen, N. Romani and G. Schuler, Cytokine gene expression in murine epidermal cell suspensions : Interleukin 1ß and macrophage inflammatory protein la are selectively expressed in Langerhans cells but are differentially regulated in culture. J. Exp. Med. 176 : 1221 (1992).PubMedCrossRefGoogle Scholar
- 4.S. Schreiber, O. Kilgus, E. Payer, R. Kutil, A. Elbe, C. Mueller and G. Stingl, Cytokine pattern of Langerhans cells isolated from murine epidermal cell cultures. J. Immunol. 149 : 3525 (1992).Google Scholar
- 8.S.B. Mizel, The Interleukins, J. FASEB, 3 : 2379 (1989).Google Scholar