Abstract
The concept of using a covalently-linked chromophoric group to ‘report’ on the environment of an enzyme’s active site was first expounded by Burr and Koshland (1964). In the work reported here, we have used 3, 4-dihydro-3-methyl-6-nitro-2H-1,3-benzoxazin-2-one (DMNB) to provide a reporter group for examining the nature of the active site in cytosolic aldehyde dehydrogenase from sheep liver.
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Kitson, T.M., Kitson, K.E. (1995). Use of a Chromophoric Reporter Group to Probe the Active Site of Cytosolic Aldehyde Dehydrogenase. In: Weiner, H., Holmes, R.S., Wermuth, B. (eds) Enzymology and Molecular Biology of Carbonyl Metabolism 5. Advances in Experimental Medicine and Biology, vol 372. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1965-2_5
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