Progress Toward the Tertiary Structure of (Class 3) Aldehyde Dehydrogenase

  • Julie Sun
  • John Hempel
  • Ronald Lindahl
  • John Perozich
  • John Rose
  • Bi-Cheng Wang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 372)

Abstract

We chose to pursue crystallography of the class 3 aldehyde dehydrogenase since, with subunits some 10% shorter than classes 1 and 2, and a dimeric rather than tetrameric quaternary structure, it presented a simpler molecule to solve. Further, despite a primary structure only some 30% identical to class 1 or 2 structures, secondary structural predictions suggested generally comparable tertiary structures (Hempel et al., 1989). The pTALDH expression system (Harper et al., 1988) provided a ready source of material. Subsequently, the observed low solubility of the enzyme (ca. lmg/ml in the presence of NAD) was probably a further advantage aiding crystallization.

Keywords

Crystallization Polyethylene Glycol Aldehyde Trypsin 

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Julie Sun
    • 1
  • John Hempel
    • 2
  • Ronald Lindahl
    • 3
  • John Perozich
    • 2
  • John Rose
    • 1
  • Bi-Cheng Wang
    • 1
  1. 1.Departments of CrystallographyUniversity of PittsburghPittsburghUSA
  2. 2.Molecular Genetics/ BiochemistryUniversity of PittsburghPittsburghUSA
  3. 3.Department of Biochemistry and Molecular BiologyUniversity of South DakotaVermillionUSA

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