Apo A-I Containing Particles and Atherosclerosis

  • Jean-Charles Fruchart
  • Graciela Castro
  • Patrick Duriez
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Epidemiological and clinical studies showing an association between decreased concentrations of high-density-lipoprotein (HDL) cholesterol and increased risk of premature coronary artery disease (CAD)1,2 have generated interest in the mechanism through which HDL prevents atherosclerosis. The HDL have been historically defined as lipoproteins with densities between 1.063 and 1.20 g/ml3. Human HDL consists of a collection of particles differing in size, density and apolipoprotein content4. Over the years, ultracentrifugation and, subsequently, polyanion precipitation and gradient gel electrophoresis have been used to fractionate HDL into subclasses5. Recognition of the importance of the apolipoproteins (apo) not only in the formation and structural stability of lipoproteins but also in their metabolism has led to the separation of HDL into further subpopulations according to their apolipoprotein composition rather than their physicochemical properties.


Cholesterol Efflux Lipoprotein Particle Peripheral Cell Adipose Cell Premature Coronary Artery Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    T. Gordon, W.P. Castelli, M.C. Hjortland, W.B. Kannel, and T.R. Dawber, High density lipoprotein as a protective factor against coronary heart disease: the Framingham Study, Am. J. Med. 62:707 (1977).PubMedCrossRefGoogle Scholar
  2. 2.
    N.E. Miller, O.H. Forte, D.S. Thelle, and O.D. Mjos, The Tromso heart study: high density lipoprotein and coronary heart disease: a prospective case control study, Lancet i:965 (1977).CrossRefGoogle Scholar
  3. 3.
    R.J. Havel, H.A. Eden, and J.H. Bragdon, The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum, J. Clin. Invest. 34:1345 (1955).PubMedCrossRefGoogle Scholar
  4. 4.
    P. Alaupovic, The physicochemical and immunological heterogeneity of human plasma high density lipoproteins, in: “Clinical and Metabolic Aspects of High-Density Lipoproteins”, N.E. Miller, G.J. Miller, eds., Elsevier Science Publishers B.V., Amsterdam (1985).Google Scholar
  5. 5.
    J.C. Fruchart, S. Marcovina, and P. Puchois, Laboratory measurements of plasma lipids and lipoproteins, in: “Human Plasma Lipoprotein”, J.C. Fruchart, J. Shepherd, eds., Walter de Gruyter, Berlin (1989).Google Scholar
  6. 6.
    M.C. Cheung, and J.J. Albers, Characterization of lipoprotein particles isolated by immunoaffmity chromatography: particles containing A-I and A-II and particles containing A-I but not A-II, J. Biol. Chem. 259:12201 (1984).PubMedGoogle Scholar
  7. 7.
    T. Ohta, S. Hattori, S. Nishiyama, and I. Matsuda, Studies on the lipid and apolipoprotein compositions of two species of apo A-I containing lipoproteins in normolipidemic males and females, J. Lipid Res. 29:721 (1988).PubMedGoogle Scholar
  8. 8.
    P. Alaupovic, E.D. Bekaert, and E. Koren, Isolation, characterization and quantitation of subclasses of plasma lipoproteins defined by their apolipoprotein composition, Atherosclerosis Rev. 20:179 (1990).Google Scholar
  9. 9.
    M.C. Cheung, A.C. Wolf, K.D. Lum, J.H. Tollefson, and J.J. Albers, Distribution and localization of lecithin: cholesteryl acyl-transferase and cholesteryl ester transfer activity in apo A-I containing lipoproteins, J. Lipid Res. 27:1135 (1986).PubMedGoogle Scholar
  10. 10.
    H.V. De Silva, W.D. Stuart, and C.R. Duvic, A 70-kDa apolipoprotein designated apo J is a marker for subclasses of human plasma high density lipoproteins, J. Biol. Chem. 265:13240 (1990).PubMedGoogle Scholar
  11. 11.
    H.B. Brewer, D. Rader, S. Fojo, and J.M. Hoeg, Frontiers in the analysis of HDL structure, function, and metabolism, in: “Disorders of HDL”, L.A. Carlson, ed., Smith-Gordon and Company Ltd, London (1990).Google Scholar
  12. 12.
    D. Rader, J.R. Schaefer, M.R. Kindt, L.A. Zecht, J.C. Fruchart, and H.B. Brewer, Differential in vivo metabolism of HDL subclasses LpA-I and LpAI:A-II in man, Clin. Res. 38:204A (1990).Google Scholar
  13. 13.
    A. Barkia, P. Puchois, N. Ghalim, G. Torpier, G. Ailhaud, and J.C. Fruchart, Differential role of apolipoprotein A-I containing particles in cholesterol efflux from adipose cells, Atherosclerosis 87:135 (1991).PubMedCrossRefGoogle Scholar
  14. 14.
    A. Steinmetz, R. Barbaras, N. Ghalim, V. Clayey, J.C. Fruchart, and G. Ailhaud, Human apolipoprotein A-N binds to apolipoprotein A-I/A-II receptor sites and promotes cholesterol efflux from adipose cells, J. Biol. Chem. 265:7859 (1990).PubMedGoogle Scholar
  15. 15.
    R. Barbaras, P. Puchois, J.C. Fruchrt, A. Pradines-Figueres, and G. Ailhaud, Purification of an apolipoprotein A binding protein from mouse adipose cells, Biochem. J. 269:767 (1990).PubMedGoogle Scholar
  16. 16.
    J.P. Slotte, J.F. Oram, and E.L. Bierman, Binding of high density lipoproteins to cell receptors promotes translocation of cholesterol from intracellular membranes to the cell surface, J. Biol. Chem. 262:12904 (1987).PubMedGoogle Scholar
  17. 17.
    N. Theret, C. Delbart, G. Aguie, J.C. Fruchart, G. Vassaux, and G. Ailhaud, Cholesterol efflux from adipose cells is coupled to diacylglycerol production and protein kinase C activation, Biochem. Biophys. Res. Commun. 173:1361 (1990).PubMedCrossRefGoogle Scholar
  18. 18.
    S. Oikawa, A.J. Mendez, M.C. Cheung, J.F. Oram, and E.L. Bierman, Effect of apo A-I and apo A-I:A-II HDL particles in intracellular cholesterol efflux, Circulation (abstract) 84(suppl II):2711 (1991).Google Scholar
  19. 19.
    W.J. Johnson, E.P.C. Kilsdonk, A. Van Tol, M.C. Phillips, and G.H. Rothblat, Cholesterol efflux from cells to immunopurified subfractions of human high density lipoprotein: LpA-I and LpA-I:A-II, J. Lipid Res. 32:1992 (1991).Google Scholar
  20. 20.
    M.C. Cheung, and J.J. Albers, Distribution of high density lipoprotein particles with different apoprotein composition: particles with A-I and A-II and particles with A-I but not A-II, J. Lipid Res. 23:747 (1982).PubMedGoogle Scholar
  21. 21.
    R.F. Atmeh, J. Shepherd, and C.J. Packard, Subpopulations of apolipoprotein A-I in human high density lipoproteins. Their metabolisms properties and response to drug therapy, Biochim. Biophys. Acta 751:175 (1983).PubMedCrossRefGoogle Scholar
  22. 22.
    E. Koren, P. Puchois, P. Alaupovic, J. Fesmire, A. Kandoussi, and J.C. Fruchart, Quantification of two types of apolipoprotein A-I containing lipoprotein particles in plasma by enzyme-linked differential antibody immunosorbent assay, Clin. Chem. 33:38 (1987).PubMedGoogle Scholar
  23. 23.
    H.J. Parra, H. Mezdour, N. Ghalim, J.M. Bard, and J.C. Fruchart, Differential electroimmunoassay of human LpA-I lipoprotein particles on ready-to-use plates, Clin. Chem. 36:1431 (1990).PubMedGoogle Scholar
  24. 24.
    G. Luc, J.M. Bard, S. Lussier-Cacan, S. Bouthillier, H.J. Parra, J.C. Fruchart, and J. Davignon, High-density lipoprotein particles in octogenarians, Metabolism 40:1238 (1991).PubMedCrossRefGoogle Scholar
  25. 25.
    P. Puchois, A. Kandoussi, P. Fiévet, J.L. Fourrier, M. Bertrand, E. Koren, and J.C. Fruchart, Apolipoprotein A-I containing lipoproteins in coronary artery disease, Atherosclerosis 68:35 (1987).PubMedCrossRefGoogle Scholar
  26. 26.
    J.J. Genest, J.M. Bard, J.C. Fruchart, J.M. Ordovas, P.F.W. Wilson, and G.J. Shaefer, Plasma apolipoproteins (a), A-I, A-II, B, E and C-III containing particles in men with premature coronary artery disease, Atherosclerosis 90:149 (1991).PubMedCrossRefGoogle Scholar
  27. 27.
    F. Cambien, H.J. Parra, D. Arveiler, J.P. Cambou, A. Evans, A. Bingham, Lipoprotein particles in patients with myocardial infarction and controls, Circulation 82(Supp1 III):348 (1990).Google Scholar
  28. 28.
    P. Amouyel, D. Isorez, J.M. Bard, M. Goldman, P. Lebel, G. Zylberberg, and J.C. Fruchart, Parenteral history of early myocardial infarction is associated with decreased levels of lipoparticle A-I in adolescents, Arterioscl. Thromb. 13:1640 (1193).CrossRefGoogle Scholar
  29. 29.
    S. Lussier-Cacan, J.M. Bard, L. Boulet, A.C. Nestruck, A.M. Grother, J.C. Fruchart, and J.C. Fruchart, and J. Davignon, Lipoprotein composition changes induced by fenofibrate in dysbetalipoproteinemia type III, Atherosclerosis 78:167 (1989).PubMedCrossRefGoogle Scholar
  30. 30.
    C. Cachera, A. Kandoussi, K. Equagoo, J.C. Fruchart, and A. Tacquet, Evaluation of apolipoprotein A-I containing particles in chronic renal failure patients undergoing hemodialysis, Am. J. Nephrol. 10:171 (1990).PubMedCrossRefGoogle Scholar
  31. 31.
    J.C. Fruchart, Insulin resistance and lipoprotein abnormalities, Diabete Metab. 17:244 (1991).PubMedGoogle Scholar
  32. 32.
    F. Fumeron, L. Brigant, H.J. Parra, J.M. Bard, J.C. Fruchart, and M. Apfelbaum, Lowering of HDL2 cholesterol and lipoprotein A-I particle levels by increasing the ratio of polyunsaturated to saturated fatty acids, Am. J. Clin. Nutr. 53:655 (1991).PubMedGoogle Scholar
  33. 33.
    P. Puchois, N. Ghalim, G. Zylberberg, P. Fiévet, C. Demarquilly, and J.C. Fruchart, Effect of alcohol intake on human apolipoprotein A-I containing lipoprotein subfractions, Arch. Intern. Med. 150:1638 (1990).PubMedCrossRefGoogle Scholar
  34. 34.
    J.M. Bard, H.J. Parra, R. Camare, G. Luc, O. Ziegler, C. Dachet, E. Bruckert, P. Douste-Blazy, P. Drouin, B. Jacotot, J.L. De Gennes, U. Keller, and J.C. Fruchart, A multicenter comparison of the effects of simvastatin and fenofibrate therapy in severe primary hypercholesterolemia, with particular emphasis on lipoproteins defined by their apolipoprotein composition, Metabolism 4:498 (1992).CrossRefGoogle Scholar
  35. 35.
    J.M. Bard, H.J. Parra, P. Douste-Blazy, and J.C. Fruchart, Effect of pravastatin, an HMG CoA reductase inhibitor, and cholestyramine, a bile acid sequestrant, on lipoprotein particles defined by their apolipoprotein composition, Metabolism 39:269 (1990).PubMedCrossRefGoogle Scholar
  36. 36.
    J.C. Fruchart, C. De Geitère, B. Delfly, G.R. Castro - Unpublished results.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Jean-Charles Fruchart
    • 1
  • Graciela Castro
    • 1
  • Patrick Duriez
    • 1
  1. 1.Institut Pasteur 1SERLIA & Unité INSERM 325Lille CédexFrance

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