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Affinity Partitioning of Metal Ions in Aqueous Biphasic Systems: Experimental and Theoretical Aspects

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Aqueous Biphasic Separations

Abstract

Even though the first report about aqueous biphasic systems appeared early in this century,1 their regular application to the purification of biomolecules did not start until 1956 when the partial isolation of organelles from cell extracts using a poly(ethylene glycol) (PEG)/potassium phosphate system, was published by Albertsson.2 Over the years, aqueous biphasic systems have been used successfully in several separation schemes and the number of applications has expanded tremendously. Most of the research has been in the separation and purification of proteins, cells, cell organelles, viruses, membrane fragments, and other biological materials.3-7 In addition, aqueous biphasic systems have been used to characterize surface properties of biomolecules such as charge and hydrophobicity.8

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Authors and Affiliations

  1. Department of Chemical and Environmental Engineering, University of Arizona, Tucson, AZ, 85721, USA

    Roberto Guzmán & Carlos M. Téllez

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  1. Roberto Guzmán
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  2. Carlos M. Téllez
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Editors and Affiliations

  1. Northern Illinois University, DeKalb, Illinois

    Robin D. Rogers

  2. University of Georgia, Athens, Georgia

    Mark A. Eiteman

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Guzmán, R., Téllez, C.M. (1995). Affinity Partitioning of Metal Ions in Aqueous Biphasic Systems: Experimental and Theoretical Aspects. In: Rogers, R.D., Eiteman, M.A. (eds) Aqueous Biphasic Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1953-9_9

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  • DOI: https://doi.org/10.1007/978-1-4615-1953-9_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5802-2

  • Online ISBN: 978-1-4615-1953-9

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